Glycoproteins and glycosylation: apolipoprotein c3 glycoforms by top-down maldi-tof mass spectrometry.

Yan Zhang, Alan R. Sinaiko, Gary L. Nelsestuen

Research output: Contribution to journalArticle

Abstract

Apolipoprotein C3 (ApoC3) is synthesized in liver so that levels or isoform distributions may constitute indicators of liver pathogenesis. The glycoforms of intact protein ApoC3 in serum or plasma can be readily analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry after a one-step extraction using a C4 reverse-phase ZipTip. Glycoform distributions were sensitive to severe systemic diseases such as sepsis or liver diseases such as chronic hepatitis C and alcoholic liver cirrhosis. Glycoisoform distributions were also altered in persons with elevated body mass index and were corrected to normal distribution by metformin, a common drug used for diabetes therapy. This simple method may offer an approach to analysis of health and the mechanism of drug therapies.

Original languageEnglish (US)
Pages (from-to)141-150
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume909
StatePublished - Dec 1 2012

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Apolipoproteins
Glycosylation
Mass Spectrometry
Glycoproteins
Alcoholic Liver Cirrhosis
Metformin
Liver
Normal Distribution
Chronic Hepatitis C
Liver Diseases
Sepsis
Protein Isoforms
Lasers
Body Mass Index
Drug Therapy
Health
Serum
Pharmaceutical Preparations
Proteins
Therapeutics

Cite this

Glycoproteins and glycosylation : apolipoprotein c3 glycoforms by top-down maldi-tof mass spectrometry. / Zhang, Yan; Sinaiko, Alan R.; Nelsestuen, Gary L.

In: Methods in molecular biology (Clifton, N.J.), Vol. 909, 01.12.2012, p. 141-150.

Research output: Contribution to journalArticle

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