TY - JOUR
T1 - Glycolipids are not extracted from phospholipid bilayers by binding to ferritin-lectin conjugates
AU - Brown, Rhoderick E.
AU - Allietta, Margaretta
AU - Tillack, Thomas W.
AU - Thompson, Thomas E.
PY - 1983/6/23
Y1 - 1983/6/23
N2 - A radioactively-labelled glycosphingolipid, asialo-GM1, has been incorporated into phosphatidylcholine multilamellar vesicles. After incubation with ferritin-Ricinus communis agglutinin 60 (RCA 60) conjugate at different temperatures, the vesicles were separated from the conjugate by discontinuous density gradient ultracentrifugation. Measurement of the distribution of the radioactively-labelled asialo-GM1 in the pelleted conjugate fraction and freeze-etch electron microscopy of the vesicle fraction indicate that the decrease in labelling of asialo-GM1-containing vesicles by ferritin-RCA 60 conjugate with increasing temperatures (Tillack, T.W., Wong, M., Allietta, M. and Thompson, T.E. (1982) Biochim. Biophys. Acta 691, 261-273) reflects a decrease in apparent binding affinity rather than an ability of the conjugate to extract glycolipid from the phospholipid bilayer after binding.
AB - A radioactively-labelled glycosphingolipid, asialo-GM1, has been incorporated into phosphatidylcholine multilamellar vesicles. After incubation with ferritin-Ricinus communis agglutinin 60 (RCA 60) conjugate at different temperatures, the vesicles were separated from the conjugate by discontinuous density gradient ultracentrifugation. Measurement of the distribution of the radioactively-labelled asialo-GM1 in the pelleted conjugate fraction and freeze-etch electron microscopy of the vesicle fraction indicate that the decrease in labelling of asialo-GM1-containing vesicles by ferritin-RCA 60 conjugate with increasing temperatures (Tillack, T.W., Wong, M., Allietta, M. and Thompson, T.E. (1982) Biochim. Biophys. Acta 691, 261-273) reflects a decrease in apparent binding affinity rather than an ability of the conjugate to extract glycolipid from the phospholipid bilayer after binding.
KW - Electron microscopy
KW - Ferritin-ricin conjugate
KW - Freeze-etching
KW - Glycosphingolipid anchoring
KW - Phosphatidylcholine bilayer
UR - http://www.scopus.com/inward/record.url?scp=25344433879&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=25344433879&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(83)90047-0
DO - 10.1016/0005-2736(83)90047-0
M3 - Article
AN - SCOPUS:25344433879
SN - 0005-2736
VL - 731
SP - 495
EP - 499
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 3
ER -