Glycolipid transfer protein from bovine brain

Martin Wong, Rhoderick E. Brown, Y. Barenholz, Thomas E. Thompson

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Glycolipid transfer protein from bovine brain has been purified partially by ammonium sulfate precipitation, CM-52 ion-exchange, and Sephadex G-75 column chromatography. Both pyrene-labeled and tritium-labeled gluco-cerebrosides have been used to study the kinetics of protein-mediated transfer between donor and acceptor vesicles. Protein accelerates glucocerebroside transfer but does not accelerate phospholipid transfer. In colyophilized small sonicated vesicles (10% glucocerebroside, 90% l-palmitoyl-2-oleoylphosphatidylcholine) about two-thirds of the glycolipid is transferred in 2 h and the remaining one-third does not transfer (up to 5 h). For donor and acceptor vesicles made of dipalmitoylphosphatidylcholine or 1-palmitoyl-2-oleoylphosphatidylcholine, glucocerebroside (10% in donors) is transferred rapidly only when both the donor and acceptor matrix phospholipids are in the liquid-crystalline state. If either donor or acceptor vesicles are in the gel state, transfer protein mediated transfer is much reduced. The amount of transfer protein bound specifically to glucocerebroside-con-taining vesicles is nearly equal above and below the matrix phospholipid phase transition temperature. Bound protein transfers glucocerebroside upon addition of acceptor vesicles.

Original languageEnglish (US)
Pages (from-to)6498-6505
Number of pages8
JournalBiochemistry
Volume23
Issue number26
DOIs
StatePublished - 1984

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Copyright 2017 Elsevier B.V., All rights reserved.

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