Glutathione disrupts galectin-10 charcot-leyden crystal formation to possibly ameliorate eosinophil-based diseases such as asthma

Heya Na, Hend Sayed, Gabriela Jaramillo Ayala, Xing Wang, Yuhan Liu, Jinyi Yu, Tianhao Liu, Kevin H. Mayo, Jiyong Su

Research output: Contribution to journalArticlepeer-review


Charcot-Leyden crystals (CLCs) are the hallmark of many eosinophilic-based diseases, such as asthma. Here, we report that reduced glutathione (GSH) disrupts CLCs and inhibits crystallization of human galectin-10 (Gal-10). GSH has no effect on CLCs from monkeys (Macaca fascicularis or M. mulatta), even though monkey Gal-10s contain Cys29 and Cys32. Interestingly, human Gal-10 contains another cysteine residue (Cys57). Because GSH cannot disrupt CLCs formed by the human Gal-10 variant C57A or inhibit its crystallization, the effects of GSH on human Gal-10 or CLCs most likely occur by chemical modification of Cys57. We further report the crystal structures of Gal- 10 from M. fascicularis and M. mulatta, along with their ability to bind to lactose and inhibit erythrocyte agglutination. Structural comparison with human Gal-10 shows that Cys57 and Gln75 within the ligand binding site are responsible for the loss of lactose binding. Pull-down experiments and mass spectrometry show that human Gal-10 interacts with tubulin α-1B, with GSH, GTP and Mg2+ stabilizing this interaction and colchicine inhibiting it. Overall, this study enhances our understanding of Gal-10 function and CLC formation and suggests that GSH may be used as a pharmaceutical agent to ameliorate CLC-induced diseases.

Original languageEnglish (US)
Pages (from-to)613-622
Number of pages10
JournalActa Biochimica et Biophysica Sinica
Issue number4
StatePublished - 2023

Bibliographical note

Funding Information:
This work was supported by the grants from the National Natural Science Foundation of China (No. 32171255) and Industrialization Cultivation Planning Project of Jilin Provincial Department of Education (No. JJKH20221168CY).

Publisher Copyright:
© 2023, Science Press. All rights reserved.


  • Asthma
  • Charcot-Leyden crystal
  • Galectin-10
  • Glutathione
  • Tubulin α-1B

PubMed: MeSH publication types

  • Journal Article


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