Glue protein production can be triggered by steroid hormone signaling independent of the developmental program in Drosophila melanogaster

Yuya Kaieda, Ryota Masuda, Ritsuo Nishida, Mary Jane Shimell, Michael B. O'Connor, Hajime Ono

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Steroid hormones regulate life stage transitions, allowing animals to appropriately follow a developmental timeline. During insect development, the steroid hormone ecdysone is synthesized and released in a regulated manner by the prothoracic gland (PG) and then hydroxylated to the active molting hormone, 20-hydroxyecdysone (20E), in peripheral tissues. We manipulated ecdysteroid titers, through temporally controlled over-expression of the ecdysteroid-inactivating enzyme, CYP18A1, in the PG using the GeneSwitch-GAL4 system in the fruit fly Drosophila melanogaster. We monitored expression of a 20E-inducible glue protein gene, Salivary gland secretion 3 (Sgs3), using a Sgs3:GFP fusion transgene. In wild type larvae, Sgs3-GFP expression is activated at the midpoint of the third larval instar stage in response to the rising endogenous level of 20E. By first knocking down endogenous 20E levels during larval development and then feeding 20E to these larvae at various stages, we found that Sgs3-GFP expression could be triggered at an inappropriate developmental stage after a certain time lag. This stage-precocious activation of Sgs3 required expression of the Broad-complex, similar to normal Sgs3 developmental regulation, and a small level of nutritional input. We suggest that these studies provide evidence for a tissue-autonomic regulatory system for a metamorphic event independent from the primary 20E driven developmental progression.

Original languageEnglish (US)
Pages (from-to)166-176
Number of pages11
JournalDevelopmental Biology
Volume430
Issue number1
DOIs
StatePublished - Oct 1 2017

Keywords

  • Broad Complex
  • Developmental checkpoint
  • Drosophila melanogaster
  • Salivary gland
  • Salivary gland secretion 3
  • Steroid hormone

Fingerprint Dive into the research topics of 'Glue protein production can be triggered by steroid hormone signaling independent of the developmental program in Drosophila melanogaster'. Together they form a unique fingerprint.

  • Cite this