TY - JOUR
T1 - Glucose metabolism in mammalian cell culture
T2 - New insights for tweaking vintage pathways
AU - Mulukutla, Bhanu Chandra
AU - Khan, Salmaan
AU - Lange, Alex
AU - Hu, Wei Shou
PY - 2010/9
Y1 - 2010/9
N2 - Cultured mammalian cells are major vehicles for producing therapeutic proteins, and energy metabolism in those cells profoundly affects process productivity. The characteristic high glucose consumption and lactate production of industrial cell lines as well as their adverse effects on productivity have been the target of both cell line and process improvement for several decades. Recent research advances have shed new light on regulation of glucose metabolism and its links to cell proliferation. This review highlights our current understanding in this area of crucial importance in bioprocessing and further discusses strategies for harnessing new findings toward process enhancement through the manipulation of cellular energy metabolism.
AB - Cultured mammalian cells are major vehicles for producing therapeutic proteins, and energy metabolism in those cells profoundly affects process productivity. The characteristic high glucose consumption and lactate production of industrial cell lines as well as their adverse effects on productivity have been the target of both cell line and process improvement for several decades. Recent research advances have shed new light on regulation of glucose metabolism and its links to cell proliferation. This review highlights our current understanding in this area of crucial importance in bioprocessing and further discusses strategies for harnessing new findings toward process enhancement through the manipulation of cellular energy metabolism.
UR - http://www.scopus.com/inward/record.url?scp=77955843732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955843732&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2010.06.005
DO - 10.1016/j.tibtech.2010.06.005
M3 - Review article
C2 - 20691487
AN - SCOPUS:77955843732
SN - 0167-7799
VL - 28
SP - 476
EP - 484
JO - Trends in biotechnology
JF - Trends in biotechnology
IS - 9
ER -