Glucose-6-phosphatase gene expression and activity are modulated in hemorrhagic shock: Evidence for a new heat-sensitive activator

Subir R. Maitra, Wansong S. Pan, Alex J. Lange, M. Raafat El-Maghrabi, Naji N. Abumrad, Simon J. Pilkis

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Decreased hepatic fructose 2,6-bisphosphate levels were observed in the early phase of hemorrhagic shock. The lower sugar bisphosphatae level was a result of increased phosphoenolpyruvate levels and decreased glucose-6-phosphate and fructose-6-phosphate levels. The decreased glucose-6-phosphate levels correlated with increased activity of liver glucose-6-phosphatase and a concomitant 2.5-fold increase in glucose-6-phosphatase mRNA abundance. In addition, protein-free filtrate from hemorrhagic shock rats, but not from control rats, increased glucose-6-phosphatase activity. However, when control and hemorrhagic shock protein-free filtrates were heated, they both increased the glucose-6-phosphatase activity of the respective microsomes to the same extent. It is concluded that the early hyperglycemic phase of hemorrhagic shock is due to enhanced glucose-6-phosphatase gene expression and activity and the generation of a heat sensitive activator of the enzyme.

Original languageEnglish (US)
Pages (from-to)716-724
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume204
Issue number2
DOIs
StatePublished - Oct 31 1994

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