GltS regulates biofilm formation in methicillin-resistant Staphylococcus aureus

Miho Shibamura-Fujiogi, Xiaogang Wang, Wiriya Maisat, Sophia Koutsogiannaki, Yunan Li, Yue Chen, Jean C. Lee, Koichi Yuki

Research output: Contribution to journalArticlepeer-review

Abstract

Biofilm-based infection is a major healthcare burden. Methicillin-resistant Staphylococcus aureus (MRSA) is one of major organisms responsible for biofilm infection. Although biofilm is induced by a number of environmental signals, the molecule responsible for environmental sensing is not well delineated. Here we examined the role of ion transporters in biofilm formation and found that the sodium-glutamate transporter gltS played an important role in biofilm formation in MRSA. This was shown by gltS transposon mutant as well as its complementation. The lack of exogenous glutamate also enhanced biofilm formation in JE2 strain. The deficiency of exogenous glutamate intake accelerated endogenous glutamate/glutamine production, which led to the activation of the urea cycle. We also showed that urea cycle activation was critical for biofilm formation. In conclusion, we showed that gltS was a critical regulator of biofilm formation by controlling the intake of exogenous glutamate. An intervention to target glutamate intake may be a potential useful approach against biofilm.

Original languageEnglish (US)
Article number1284
JournalCommunications biology
Volume5
Issue number1
DOIs
StatePublished - Dec 2022

Bibliographical note

Funding Information:
We acknowledge Lifei Hou, Mariel Manzol, and Marie Bermudez (all Boston Children’s Hospital) for discussion.

Publisher Copyright:
© 2022, The Author(s).

PubMed: MeSH publication types

  • Journal Article

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