Glomerular cell number in normal subjects and in type 1 diabetic patients

Michael W. Steffes, Derek Schmidt, Rebecca McCrery, John M. Basgen, Keith Drummond, Christine Aebi, Mimi Belmonte, Robert Gardiner, Paul Goodyear, Michael Kramer, Constantin Polychronakos, Samy Suissz, Diane Laforte, Jan Braaten, Kenneth Fautht, Khalil Khoury, Paul Czernichow, Veronique Beaudoin, Helen Beaufils, Claire Levy-Marchal & 17 others Marie Claire Gubler, Phillippe Passa, Dominique Simon, Michael Mauer, Blanche Chavers, Thomas Hostetter, Youngki Kim, Connie Manske, Alfred Michael, Krisha Saxena, Alan Sinaiko, Michael Steffes, Robert Vernier, Trudy Strand, Richard Bergenstal, Joseph Sockalosky, Stephen Rich

Research output: Contribution to journalArticle

291 Citations (Scopus)

Abstract

Background. The number of cells in glomeruli has been a challenging measure, especially in human kidneys, with only a small amount of tissue obtained by biopsy. However, the number of cells and their function are important determinants of renal function in health and disease. Methods. Modern morphometric techniques have now provided the means to determine the numerical density (Nv) and number (with a measure of glomerular volume) of endothelial cells, mesangial cells, and podocytes in plastic-embedded renal tissue biopsied from nondiabetic subjects (N = 36) and type 1 diabetic patients (N = 46) over an extended age range from childhood through late adult. Results. Nv values for all glomerular cells varied only slightly with age and did not change within the range of glomerular lesions of diabetes studied. Thus, the increase in glomerular volume during childhood to a steady level thereafter was the primary determinant of total glomerular cell number. The number of mesangial cells and endothelial cells increased with age, reflecting the increase in all cells, while the podocytes remained unchanged in number over all ages studied (10 to 69 years). Numbers of total glomerular cells, mesangial cells, and endothelial cells were not changed with diabetes, while podocytes were fewer in number in diabetic patients of all ages, with reduced podocyte numbers even in diabetes of short duration. Conclusions. The essentially constant glomerular cell density in nondiabetic and diabetic subjects under different circumstances possibly indicates an underlying propensity for the glomerulus to regulate its architecture to maintain a constant number of cells per volume, no matter the size of the glomerulus or the severity of diabetic nephropathy studied in this set of patients. The reductions in podocyte numbers in both younger and older diabetic patients indicate a significant risk for functional abnormalities as diabetic nephropathy progresses. Moreover, these observations do not support the suggestion of marked increases in glomerular cell number (and especially mesangial cells) with the development and progression of diabetic nephropathy.

Original languageEnglish (US)
Pages (from-to)2104-2113
Number of pages10
JournalKidney international
Volume59
Issue number6
DOIs
StatePublished - Jan 1 2001

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Podocytes
Mesangial Cells
Cell Count
Diabetic Nephropathies
Endothelial Cells
Kidney
Cell Size
Plastics
Biopsy
Health

Keywords

  • Cell density
  • Diabetic nephropathy
  • Morphometry
  • Podocytes
  • Progressive renal disease
  • Renal function
  • Urine volume

Cite this

Steffes, M. W., Schmidt, D., McCrery, R., Basgen, J. M., Drummond, K., Aebi, C., ... Rich, S. (2001). Glomerular cell number in normal subjects and in type 1 diabetic patients. Kidney international, 59(6), 2104-2113. https://doi.org/10.1046/j.1523-1755.2001.0590062104.x

Glomerular cell number in normal subjects and in type 1 diabetic patients. / Steffes, Michael W.; Schmidt, Derek; McCrery, Rebecca; Basgen, John M.; Drummond, Keith; Aebi, Christine; Belmonte, Mimi; Gardiner, Robert; Goodyear, Paul; Kramer, Michael; Polychronakos, Constantin; Suissz, Samy; Laforte, Diane; Braaten, Jan; Fautht, Kenneth; Khoury, Khalil; Czernichow, Paul; Beaudoin, Veronique; Beaufils, Helen; Levy-Marchal, Claire; Gubler, Marie Claire; Passa, Phillippe; Simon, Dominique; Mauer, Michael; Chavers, Blanche; Hostetter, Thomas; Kim, Youngki; Manske, Connie; Michael, Alfred; Saxena, Krisha; Sinaiko, Alan; Steffes, Michael; Vernier, Robert; Strand, Trudy; Bergenstal, Richard; Sockalosky, Joseph; Rich, Stephen.

In: Kidney international, Vol. 59, No. 6, 01.01.2001, p. 2104-2113.

Research output: Contribution to journalArticle

Steffes, MW, Schmidt, D, McCrery, R, Basgen, JM, Drummond, K, Aebi, C, Belmonte, M, Gardiner, R, Goodyear, P, Kramer, M, Polychronakos, C, Suissz, S, Laforte, D, Braaten, J, Fautht, K, Khoury, K, Czernichow, P, Beaudoin, V, Beaufils, H, Levy-Marchal, C, Gubler, MC, Passa, P, Simon, D, Mauer, M, Chavers, B, Hostetter, T, Kim, Y, Manske, C, Michael, A, Saxena, K, Sinaiko, A, Steffes, M, Vernier, R, Strand, T, Bergenstal, R, Sockalosky, J & Rich, S 2001, 'Glomerular cell number in normal subjects and in type 1 diabetic patients', Kidney international, vol. 59, no. 6, pp. 2104-2113. https://doi.org/10.1046/j.1523-1755.2001.0590062104.x
Steffes, Michael W. ; Schmidt, Derek ; McCrery, Rebecca ; Basgen, John M. ; Drummond, Keith ; Aebi, Christine ; Belmonte, Mimi ; Gardiner, Robert ; Goodyear, Paul ; Kramer, Michael ; Polychronakos, Constantin ; Suissz, Samy ; Laforte, Diane ; Braaten, Jan ; Fautht, Kenneth ; Khoury, Khalil ; Czernichow, Paul ; Beaudoin, Veronique ; Beaufils, Helen ; Levy-Marchal, Claire ; Gubler, Marie Claire ; Passa, Phillippe ; Simon, Dominique ; Mauer, Michael ; Chavers, Blanche ; Hostetter, Thomas ; Kim, Youngki ; Manske, Connie ; Michael, Alfred ; Saxena, Krisha ; Sinaiko, Alan ; Steffes, Michael ; Vernier, Robert ; Strand, Trudy ; Bergenstal, Richard ; Sockalosky, Joseph ; Rich, Stephen. / Glomerular cell number in normal subjects and in type 1 diabetic patients. In: Kidney international. 2001 ; Vol. 59, No. 6. pp. 2104-2113.
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T1 - Glomerular cell number in normal subjects and in type 1 diabetic patients

AU - Steffes, Michael W.

AU - Schmidt, Derek

AU - McCrery, Rebecca

AU - Basgen, John M.

AU - Drummond, Keith

AU - Aebi, Christine

AU - Belmonte, Mimi

AU - Gardiner, Robert

AU - Goodyear, Paul

AU - Kramer, Michael

AU - Polychronakos, Constantin

AU - Suissz, Samy

AU - Laforte, Diane

AU - Braaten, Jan

AU - Fautht, Kenneth

AU - Khoury, Khalil

AU - Czernichow, Paul

AU - Beaudoin, Veronique

AU - Beaufils, Helen

AU - Levy-Marchal, Claire

AU - Gubler, Marie Claire

AU - Passa, Phillippe

AU - Simon, Dominique

AU - Mauer, Michael

AU - Chavers, Blanche

AU - Hostetter, Thomas

AU - Kim, Youngki

AU - Manske, Connie

AU - Michael, Alfred

AU - Saxena, Krisha

AU - Sinaiko, Alan

AU - Steffes, Michael

AU - Vernier, Robert

AU - Strand, Trudy

AU - Bergenstal, Richard

AU - Sockalosky, Joseph

AU - Rich, Stephen

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KW - Cell density

KW - Diabetic nephropathy

KW - Morphometry

KW - Podocytes

KW - Progressive renal disease

KW - Renal function

KW - Urine volume

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