A glass carbon (GC) electrode has been modified with horseradish peroxidase (HRP) molecules, which are immobilized on the partially reduced graphene oxide (PCRG). The surface properties of the as-modified electrode are characterized with scanning electron microscopy (SEM), the electrochemical characteristics of the as-modified electrode are studied using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). We demonstrated that the PCRG can promote the electron transfer between HRP and GC electrode, and the immobilized HRP maintained its catalytic activity of the decomposition of phenol and p-chlorophenol. The GC electrode modified with PCRG immobilized HRP exhibits better electrochemical property over CRG, the modified electrode may find practical application as enzyme-based amperometric sensors used for detections of phenolic molecules or other permanent organic pollutants in water. The method provides a strategy for preparation of a sensitive amperometric sensor for the detection of phenolic compounds and other permanent organic pollutants.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Electroanalytical Chemistry|
|State||Published - Aug 1 2012|
Bibliographical noteFunding Information:
This work was financially supported by the NSFC (No. 91123011, 90923041, 31070742), Shanghai Committee of Science and Technology (Grant 11DZ2260600 ), and the National “973” Program (2010CB933900) of China.
- Glass carbon electrode
- Horseradish peroxidase
- Partially reduced graphene oxide
- Phenolic compounds