Ginseng-derived type I rhamnogalacturonan polysaccharide binds to galectin-8 and antagonizes its function

Yi Zheng, Yunlong Si, Xuejiao Xu, Hongming Gu, Zhen He, Zihan Zhao, Zhangkai Feng, Jiyong Su, Kevin H. Mayo, Yifa Zhou, Guihua Tai

Research output: Contribution to journalArticlepeer-review


Background: Panax ginseng Meyer polysaccharides exhibit various biological functions, like antagonizing galectin-3-mediated cell adhesion and migration. Galectin-8 (Gal-8), with its linker-joined N- and C-terminal carbohydrate recognition domains (CRDs), is also crucial to these biological processes, and thus plays a role in various pathological disorders. Yet the effect of ginseng-derived polysaccharides in modulating Gal-8 function has remained unclear. Methods: P. ginseng-derived pectin was chromatographically isolated and enzymatically digested to obtain a series of polysaccharides. Biolayer Interferometry (BLI) quantified their binding affinity to Gal-8, and their inhibitory effects on Gal-8 was assessed by hemagglutination, cell migration and T-cell apoptosis. Results: Our ginseng-derived pectin polysaccharides consist mostly of rhamnogalacturonan-I (RG-I) and homogalacturonan (HG). BLI shows that Gal-8 binding rests primarily in RG-I and its β-1,4-galactan side chains, with sub-micromolar KD values. Both N- and C-terminal Gal-8 CRDs bind RG-I, with binding correlated with Gal-8-mediated function. Conclusion: P. ginseng RG-I pectin β-1,4-galactan side chains are crucial to binding Gal-8 and antagonizing its function. This study enhances our understanding of galectin-sugar interactions, information that may be used in the development of pharmaceutical agents targeting Gal-8.

Original languageEnglish (US)
Pages (from-to)202-210
Number of pages9
JournalJournal of Ginseng Research
Issue number2
StatePublished - Mar 2024

Bibliographical note

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  • Galactan
  • Galectin-8
  • Interaction
  • Pectin
  • Rhamnogalacturonan I


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