Gibberellin biosynthesis in bacteria: Separate ent-copalyl diphosphate and ent-kaurene synthases in Bradyrhizobium japonicum

Dana Morrone; Jacob Chambers; Luke Lowry; Gunjune Kim; Aldwin Anterola; Kelly Bender; Reuben J. Peters

doi:10.1016/j.febslet.2008.12.052

Gibberellin biosynthesis in bacteria: Separate ent-copalyl diphosphate and ent-kaurene synthases in Bradyrhizobium japonicum

Dana Morrone, Jacob Chambers, Luke Lowry, Gunjune Kim, Aldwin Anterola, Kelly Bender, Reuben J. Peters

Research output: Contribution to journal › Article › peer-review

147 Scopus citations

Abstract

Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional diterpene synthase. We demonstrate here that Bradyrhizobium japonicum encodes separate ent-copalyl diphosphate and ent-kaurene synthases. These are found in an operon whose enzymatic composition indicates that gibberellin biosynthesis in bacteria represents a third independently assembled pathway relative to plants and fungi. Nevertheless, sequence comparisons also suggest potential homology between diterpene synthases from bacteria, plants, and fungi.

Original language	English (US)
Pages (from-to)	475-480
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	2
DOIs	https://doi.org/10.1016/j.febslet.2008.12.052
State	Published - Jan 22 2009
Externally published	Yes

Bibliographical note

Funding Information:
We thank Dr. Michael Sadowsky (University of Minnesota) for the Bradyrhizobium japonicum strain USDA110 used in this study and Meimei Xu for constructing the pGG-DEST vector. This work was funded by SEED grants from Southern Illinois University to A.A. and K.B., and a National Science Foundation grant to R.J.P. (MCB-0416948).

Keywords

Bradyrhizobium japonicum
Copalyl diphosphate synthase
Diterpene
Gibberellin
Terpene synthase
ent-Kaurene synthase

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10.1016/j.febslet.2008.12.052

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abstract = "Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional diterpene synthase. We demonstrate here that Bradyrhizobium japonicum encodes separate ent-copalyl diphosphate and ent-kaurene synthases. These are found in an operon whose enzymatic composition indicates that gibberellin biosynthesis in bacteria represents a third independently assembled pathway relative to plants and fungi. Nevertheless, sequence comparisons also suggest potential homology between diterpene synthases from bacteria, plants, and fungi.",

keywords = "Bradyrhizobium japonicum, Copalyl diphosphate synthase, Diterpene, Gibberellin, Terpene synthase, ent-Kaurene synthase",

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note = "Funding Information: We thank Dr. Michael Sadowsky (University of Minnesota) for the Bradyrhizobium japonicum strain USDA110 used in this study and Meimei Xu for constructing the pGG-DEST vector. This work was funded by SEED grants from Southern Illinois University to A.A. and K.B., and a National Science Foundation grant to R.J.P. (MCB-0416948). ",

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AU - Lowry, Luke

AU - Kim, Gunjune

AU - Anterola, Aldwin

AU - Bender, Kelly

AU - Peters, Reuben J.

N1 - Funding Information: We thank Dr. Michael Sadowsky (University of Minnesota) for the Bradyrhizobium japonicum strain USDA110 used in this study and Meimei Xu for constructing the pGG-DEST vector. This work was funded by SEED grants from Southern Illinois University to A.A. and K.B., and a National Science Foundation grant to R.J.P. (MCB-0416948).

PY - 2009/1/22

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N2 - Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional diterpene synthase. We demonstrate here that Bradyrhizobium japonicum encodes separate ent-copalyl diphosphate and ent-kaurene synthases. These are found in an operon whose enzymatic composition indicates that gibberellin biosynthesis in bacteria represents a third independently assembled pathway relative to plants and fungi. Nevertheless, sequence comparisons also suggest potential homology between diterpene synthases from bacteria, plants, and fungi.

AB - Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional diterpene synthase. We demonstrate here that Bradyrhizobium japonicum encodes separate ent-copalyl diphosphate and ent-kaurene synthases. These are found in an operon whose enzymatic composition indicates that gibberellin biosynthesis in bacteria represents a third independently assembled pathway relative to plants and fungi. Nevertheless, sequence comparisons also suggest potential homology between diterpene synthases from bacteria, plants, and fungi.

KW - Bradyrhizobium japonicum

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KW - Diterpene

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KW - Terpene synthase

KW - ent-Kaurene synthase

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Gibberellin biosynthesis in bacteria: Separate ent-copalyl diphosphate and ent-kaurene synthases in Bradyrhizobium japonicum

Abstract

Bibliographical note

Keywords

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