Gentle dielectrophoretic focusing of yeast cells in curved microchannels

Christopher Church; Gaoyan Wang; Junjie Zhu; Tzuen Rong Jeremy Tzeng; Xiangchun Xuan

doi:10.1115/MNHMT2009-18489

Gentle dielectrophoretic focusing of yeast cells in curved microchannels

Christopher Church, Gaoyan Wang, Junjie Zhu, Tzuen Rong Jeremy Tzeng, Xiangchun Xuan

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Focusing cells into a tight stream is usually a necessary step prior to counting, detecting and sorting them in, for example, microfluidic flow cytometers. We present herein a simple and gentle cell focusing technique in physiological solutions through a serpentine microchannel using DC-biased AC electric fields. This electrokinetic focusing eliminates sheath flows and in-channel microelectrodes. It results from the cross-stream dielectrophoretic motion of cells induced by the intrinsic channel curvatures. The effects of electric field magnitude, AC to DC electric field ratio, AC field frequency, and cell concentration on the focusing performance of yeast cells will be studied.

Original language	English (US)
Title of host publication	Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Pages	283-287
Number of pages	5
DOIs	https://doi.org/10.1115/MNHMT2009-18489
State	Published - 2010
Externally published	Yes
Event	ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 - Shanghai, China Duration: Dec 18 2009 → Dec 21 2009

Publication series

Name	Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Volume	1

Other

Other	ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Country/Territory	China
City	Shanghai
Period	12/18/09 → 12/21/09

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10.1115/MNHMT2009-18489

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Church, C., Wang, G., Zhu, J., Tzeng, T. R. J., & Xuan, X. (2010). Gentle dielectrophoretic focusing of yeast cells in curved microchannels. In Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 (pp. 283-287). (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; Vol. 1). https://doi.org/10.1115/MNHMT2009-18489

Gentle dielectrophoretic focusing of yeast cells in curved microchannels. / Church, Christopher; Wang, Gaoyan; Zhu, Junjie et al.
Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. p. 283-287 (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Church, C, Wang, G, Zhu, J, Tzeng, TRJ & Xuan, X 2010, Gentle dielectrophoretic focusing of yeast cells in curved microchannels. in Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, vol. 1, pp. 283-287, ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, Shanghai, China, 12/18/09. https://doi.org/10.1115/MNHMT2009-18489

Church C, Wang G, Zhu J, Tzeng TRJ, Xuan X. Gentle dielectrophoretic focusing of yeast cells in curved microchannels. In Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. p. 283-287. (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009). doi: 10.1115/MNHMT2009-18489

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abstract = "Focusing cells into a tight stream is usually a necessary step prior to counting, detecting and sorting them in, for example, microfluidic flow cytometers. We present herein a simple and gentle cell focusing technique in physiological solutions through a serpentine microchannel using DC-biased AC electric fields. This electrokinetic focusing eliminates sheath flows and in-channel microelectrodes. It results from the cross-stream dielectrophoretic motion of cells induced by the intrinsic channel curvatures. The effects of electric field magnitude, AC to DC electric field ratio, AC field frequency, and cell concentration on the focusing performance of yeast cells will be studied.",

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AB - Focusing cells into a tight stream is usually a necessary step prior to counting, detecting and sorting them in, for example, microfluidic flow cytometers. We present herein a simple and gentle cell focusing technique in physiological solutions through a serpentine microchannel using DC-biased AC electric fields. This electrokinetic focusing eliminates sheath flows and in-channel microelectrodes. It results from the cross-stream dielectrophoretic motion of cells induced by the intrinsic channel curvatures. The effects of electric field magnitude, AC to DC electric field ratio, AC field frequency, and cell concentration on the focusing performance of yeast cells will be studied.

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Gentle dielectrophoretic focusing of yeast cells in curved microchannels

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