Genotype Diversity of Puroindoline Genes (Pina-D1 and Pinb-D1) in Bread Wheat Cultivars Developed in Iran and CIMMYT

Mohsen Mohammadi, Elham Mehrazar, Ali Izadi-Darbandi, Goodarz Najafian

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Grain hardness has a considerable effect on milling yield, particle size, and water absorption of wheat (Triticum aestivum L.) flour and is considered an integral trait in wheat end-use classification. Kernel hardness in wheat is controlled by the Hardness locus on chromosome 5D. In this study, we investigated genotypic variation of genes involved in grain hardness in wheat cultivars developed in Iran or CIMMYT. We have used two allele-specific PCR marker methodologies to identify allelic variation of Pina-D1 and Pinb-D1 genes. We also determined the force required for seed rupture by compression testing (CT) in selected number of cultivars. Two alleles (Pina-D1a and Pina-D1b) for Pina-D1 and three alleles (Pinb-D1a, Pinb-D1b, and Pinb-D1d) for Pinb-D1 were detected. The most common genotype observed was Pina-D1a/Pinb-D1a. The second most frequent genotype was Pina-D1b/Pinb-D1a. Genotypes Pina-D1a/Pinb-D1d and Pina-D1b/Pinb-D1d were also observed but at very low frequencies. In general, compression testing data were in agreement with the grain hardness expected from each genotypic class. Most Pina-D1b-carrying germplasm were those from the CIMMYT global wheat program. The allele Pinb-D1d was found in combination with Pina-D1a and with Pina-D1b. The allele Pinb-D1d did not increase grain hardness as expected.

Original languageEnglish (US)
Pages (from-to)361-375
Number of pages15
JournalJournal of Crop Improvement
Volume27
Issue number4
DOIs
StatePublished - Jul 8 2013

Keywords

  • allele-specific markers
  • compression test
  • end-use quality traits
  • hardness locus
  • kernel hardness

Fingerprint Dive into the research topics of 'Genotype Diversity of Puroindoline Genes (Pina-D1 and Pinb-D1) in Bread Wheat Cultivars Developed in Iran and CIMMYT'. Together they form a unique fingerprint.

  • Cite this