Genomic structure, promoter identification and chromosomal mapping of a mouse nuclear orphan receptor expressed in embryos and adult testes

Chih Hao Lee; Li Na Wei

Genomic structure, promoter identification and chromosomal mapping of a mouse nuclear orphan receptor expressed in embryos and adult testes

Chih Hao Lee
, Li Na Wei

Pharmacology

Research output: Contribution to journal › Article › peer-review

Abstract

We have isolated and characterized overlapping clones containing the complete transcribed region of a newly isolated mouse cDNA encoding an orphan receptor that has shown high degree of homology to both human and rat orphan receptors Tr2-ll and is expressed specifically in midgestation embryo and adult testis. This gene spans a distance of more than 50 kb and is organized into 13 exons. The transcription initiation site is located at the 158th nucleotide upstream of the translation initiation codon. All the exon/intron junction sequences follow the GT/AG rule. Based upon Northern blot analysis and the size of transcribed region of the gene, its transcript was determined to be approximately 2.5 kb in length. Within approximately 500 bp upstream of the transcription initiation site, several immune response regulatory elements were identified but no TATA box was located. This gene was mapped to the distal region of mouse chromosome 10 and its locus has been designated as Tr2-l 1. Immunohistochemical studies show that the Tr2-ll protein is present mainly in advanced germ cell populations of mature testes and Tr2-11 gene expression is dramatically decreased in vitamin A-depleted animals.

Original language	English (US)
Pages (from-to)	A444
Journal	FASEB Journal
Volume	10
Issue number	3
State	Published - 1996

Bibliographical note

Funding Information:
This work was supported by Grants DK46866±01 and SMF-975± 95 and a Grant-in-Aid of Research, Artistry, and Scholarship from the Graduate School of the University of Minnesota to L.-N.W. and in part by the National Cancer Institute, DHHS, under Contract N01-CO-46000 with ABL. We thank Dr. Liming Chang for help in antibody puri®cation and Debbie Barnhart for excellent technical assistance. We thank Dr. K. P. Roberts for a critical reading of the manuscript. We also thank Drs. D. Hamilton, K. P. Roberts, and J. E. Siiteri for their help in testis immunohistochemistry.

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title = "Genomic structure, promoter identification and chromosomal mapping of a mouse nuclear orphan receptor expressed in embryos and adult testes",

abstract = "We have isolated and characterized overlapping clones containing the complete transcribed region of a newly isolated mouse cDNA encoding an orphan receptor that has shown high degree of homology to both human and rat orphan receptors Tr2-ll and is expressed specifically in midgestation embryo and adult testis. This gene spans a distance of more than 50 kb and is organized into 13 exons. The transcription initiation site is located at the 158th nucleotide upstream of the translation initiation codon. All the exon/intron junction sequences follow the GT/AG rule. Based upon Northern blot analysis and the size of transcribed region of the gene, its transcript was determined to be approximately 2.5 kb in length. Within approximately 500 bp upstream of the transcription initiation site, several immune response regulatory elements were identified but no TATA box was located. This gene was mapped to the distal region of mouse chromosome 10 and its locus has been designated as Tr2-l 1. Immunohistochemical studies show that the Tr2-ll protein is present mainly in advanced germ cell populations of mature testes and Tr2-11 gene expression is dramatically decreased in vitamin A-depleted animals.",

author = "Lee, \{Chih Hao\} and Wei, \{Li Na\}",

note = "Funding Information: This work was supported by Grants DK46866±01 and SMF-975± 95 and a Grant-in-Aid of Research, Artistry, and Scholarship from the Graduate School of the University of Minnesota to L.-N.W. and in part by the National Cancer Institute, DHHS, under Contract N01-CO-46000 with ABL. We thank Dr. Liming Chang for help in antibody puri{\textregistered}cation and Debbie Barnhart for excellent technical assistance. We thank Dr. K. P. Roberts for a critical reading of the manuscript. We also thank Drs. D. Hamilton, K. P. Roberts, and J. E. Siiteri for their help in testis immunohistochemistry.",

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AU - Wei, Li Na

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PY - 1996

Y1 - 1996

N2 - We have isolated and characterized overlapping clones containing the complete transcribed region of a newly isolated mouse cDNA encoding an orphan receptor that has shown high degree of homology to both human and rat orphan receptors Tr2-ll and is expressed specifically in midgestation embryo and adult testis. This gene spans a distance of more than 50 kb and is organized into 13 exons. The transcription initiation site is located at the 158th nucleotide upstream of the translation initiation codon. All the exon/intron junction sequences follow the GT/AG rule. Based upon Northern blot analysis and the size of transcribed region of the gene, its transcript was determined to be approximately 2.5 kb in length. Within approximately 500 bp upstream of the transcription initiation site, several immune response regulatory elements were identified but no TATA box was located. This gene was mapped to the distal region of mouse chromosome 10 and its locus has been designated as Tr2-l 1. Immunohistochemical studies show that the Tr2-ll protein is present mainly in advanced germ cell populations of mature testes and Tr2-11 gene expression is dramatically decreased in vitamin A-depleted animals.

AB - We have isolated and characterized overlapping clones containing the complete transcribed region of a newly isolated mouse cDNA encoding an orphan receptor that has shown high degree of homology to both human and rat orphan receptors Tr2-ll and is expressed specifically in midgestation embryo and adult testis. This gene spans a distance of more than 50 kb and is organized into 13 exons. The transcription initiation site is located at the 158th nucleotide upstream of the translation initiation codon. All the exon/intron junction sequences follow the GT/AG rule. Based upon Northern blot analysis and the size of transcribed region of the gene, its transcript was determined to be approximately 2.5 kb in length. Within approximately 500 bp upstream of the transcription initiation site, several immune response regulatory elements were identified but no TATA box was located. This gene was mapped to the distal region of mouse chromosome 10 and its locus has been designated as Tr2-l 1. Immunohistochemical studies show that the Tr2-ll protein is present mainly in advanced germ cell populations of mature testes and Tr2-11 gene expression is dramatically decreased in vitamin A-depleted animals.

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Genomic structure, promoter identification and chromosomal mapping of a mouse nuclear orphan receptor expressed in embryos and adult testes

Abstract

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