TY - JOUR
T1 - Genomic structure of the mouse δ opioid receptor gene
AU - Augustin, Lance B.
AU - Felsheim, Roderick F.
AU - Min, Bon H.
AU - Fuchs, Stephanie M.
AU - Fuchs, James A.
AU - Loh, Horace H.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1995/2/6
Y1 - 1995/2/6
N2 - Using mouse δ opioid receptor (DOR) cDNA sequence to probe genomic libraries in bacteriophage λ and P1 vectors, clones traversing the entire DOR coding sequence and 5′ and 3′ flanking regions were isolated. Genomic sequence encoding mature DOR message, including 5′ and 3′ untranslated sequence, is divided by two introns of 26 kb and 3 kb, resulting in the gene occupying 32 kb of chromosomal DNA. Multiple putative transcription initiation sites were located, by RNase protection assay, in TATA-less G+C rich sequence between 390 and 140 nucleotides upstream from the ATG translation start codon. A polyadenylation site was located 1.24 kb downstream from the TGA translation stop codon. Examination of 1.3 kb of 5′ flanking sequence revealed potential binding sites for several known transcription factors including: Sp1, Ap-2, NF-κB, NF-IL6, and NGFI-B.
AB - Using mouse δ opioid receptor (DOR) cDNA sequence to probe genomic libraries in bacteriophage λ and P1 vectors, clones traversing the entire DOR coding sequence and 5′ and 3′ flanking regions were isolated. Genomic sequence encoding mature DOR message, including 5′ and 3′ untranslated sequence, is divided by two introns of 26 kb and 3 kb, resulting in the gene occupying 32 kb of chromosomal DNA. Multiple putative transcription initiation sites were located, by RNase protection assay, in TATA-less G+C rich sequence between 390 and 140 nucleotides upstream from the ATG translation start codon. A polyadenylation site was located 1.24 kb downstream from the TGA translation stop codon. Examination of 1.3 kb of 5′ flanking sequence revealed potential binding sites for several known transcription factors including: Sp1, Ap-2, NF-κB, NF-IL6, and NGFI-B.
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U2 - 10.1006/bbrc.1995.1160
DO - 10.1006/bbrc.1995.1160
M3 - Article
C2 - 7857252
AN - SCOPUS:0028919134
SN - 0006-291X
VL - 207
SP - 111
EP - 119
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -