Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana

Brett Lahner, Jiming Gong, Mehrzad Mahmoudian, Ellen L. Smith, Khush B. Abid, Elizabeth E. Rogers, Mary L. Guerinot, Jeffrey F. Harper, John M. Ward, Lauren McIntyre, Julian I. Schroeder, David E. Salt

Research output: Contribution to journalArticle

269 Citations (Scopus)

Abstract

Understanding the functional connections between genes, proteins, metabolites and mineral ions is one of biology's greatest challenges in the postgenomic era. We describe here the use of mineral nutrient and trace element profiling as a tool to determine the biological significance of connections between a plant's genome and its elemental profile. Using inductively coupled plasma spectroscopy, we quantified 18 elements, including essential macro- and micronutrients and various nonessential elements, in shoots of 6,000 mutagenized M2 Arabidopsis thaliana plants. We isolated 51 mutants with altered elemental profiles. One mutant contains a deletion in FRD3, a gene known to control iron-deficiency responses in A. thaliana. Based on the frequency of elemental profile mutations, we estimate 2-4% of the A. thaliana genome is involved in regulating the plant's nutrient and trace element content. These results demonstrate the utility of elemental profiling as a useful functional genomics tool.

Original languageEnglish (US)
Pages (from-to)1215-1221
Number of pages7
JournalNature biotechnology
Volume21
Issue number10
DOIs
StatePublished - Oct 1 2003

Fingerprint

Trace Elements
Trace elements
Arabidopsis
Nutrients
Genes
Food
Minerals
Plant Genome
Micronutrients
Inductively coupled plasma
Metabolites
Genomics
Macros
Spectrum Analysis
Iron
Spectroscopy
Genome
Ions
Mutation
Proteins

Cite this

Lahner, B., Gong, J., Mahmoudian, M., Smith, E. L., Abid, K. B., Rogers, E. E., ... Salt, D. E. (2003). Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana. Nature biotechnology, 21(10), 1215-1221. https://doi.org/10.1038/nbt865

Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana. / Lahner, Brett; Gong, Jiming; Mahmoudian, Mehrzad; Smith, Ellen L.; Abid, Khush B.; Rogers, Elizabeth E.; Guerinot, Mary L.; Harper, Jeffrey F.; Ward, John M.; McIntyre, Lauren; Schroeder, Julian I.; Salt, David E.

In: Nature biotechnology, Vol. 21, No. 10, 01.10.2003, p. 1215-1221.

Research output: Contribution to journalArticle

Lahner, B, Gong, J, Mahmoudian, M, Smith, EL, Abid, KB, Rogers, EE, Guerinot, ML, Harper, JF, Ward, JM, McIntyre, L, Schroeder, JI & Salt, DE 2003, 'Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana', Nature biotechnology, vol. 21, no. 10, pp. 1215-1221. https://doi.org/10.1038/nbt865
Lahner B, Gong J, Mahmoudian M, Smith EL, Abid KB, Rogers EE et al. Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana. Nature biotechnology. 2003 Oct 1;21(10):1215-1221. https://doi.org/10.1038/nbt865
Lahner, Brett ; Gong, Jiming ; Mahmoudian, Mehrzad ; Smith, Ellen L. ; Abid, Khush B. ; Rogers, Elizabeth E. ; Guerinot, Mary L. ; Harper, Jeffrey F. ; Ward, John M. ; McIntyre, Lauren ; Schroeder, Julian I. ; Salt, David E. / Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana. In: Nature biotechnology. 2003 ; Vol. 21, No. 10. pp. 1215-1221.
@article{052c60d83e284685b5bcfbd3be61d02c,
title = "Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana",
abstract = "Understanding the functional connections between genes, proteins, metabolites and mineral ions is one of biology's greatest challenges in the postgenomic era. We describe here the use of mineral nutrient and trace element profiling as a tool to determine the biological significance of connections between a plant's genome and its elemental profile. Using inductively coupled plasma spectroscopy, we quantified 18 elements, including essential macro- and micronutrients and various nonessential elements, in shoots of 6,000 mutagenized M2 Arabidopsis thaliana plants. We isolated 51 mutants with altered elemental profiles. One mutant contains a deletion in FRD3, a gene known to control iron-deficiency responses in A. thaliana. Based on the frequency of elemental profile mutations, we estimate 2-4{\%} of the A. thaliana genome is involved in regulating the plant's nutrient and trace element content. These results demonstrate the utility of elemental profiling as a useful functional genomics tool.",
author = "Brett Lahner and Jiming Gong and Mehrzad Mahmoudian and Smith, {Ellen L.} and Abid, {Khush B.} and Rogers, {Elizabeth E.} and Guerinot, {Mary L.} and Harper, {Jeffrey F.} and Ward, {John M.} and Lauren McIntyre and Schroeder, {Julian I.} and Salt, {David E.}",
year = "2003",
month = "10",
day = "1",
doi = "10.1038/nbt865",
language = "English (US)",
volume = "21",
pages = "1215--1221",
journal = "Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana

AU - Lahner, Brett

AU - Gong, Jiming

AU - Mahmoudian, Mehrzad

AU - Smith, Ellen L.

AU - Abid, Khush B.

AU - Rogers, Elizabeth E.

AU - Guerinot, Mary L.

AU - Harper, Jeffrey F.

AU - Ward, John M.

AU - McIntyre, Lauren

AU - Schroeder, Julian I.

AU - Salt, David E.

PY - 2003/10/1

Y1 - 2003/10/1

N2 - Understanding the functional connections between genes, proteins, metabolites and mineral ions is one of biology's greatest challenges in the postgenomic era. We describe here the use of mineral nutrient and trace element profiling as a tool to determine the biological significance of connections between a plant's genome and its elemental profile. Using inductively coupled plasma spectroscopy, we quantified 18 elements, including essential macro- and micronutrients and various nonessential elements, in shoots of 6,000 mutagenized M2 Arabidopsis thaliana plants. We isolated 51 mutants with altered elemental profiles. One mutant contains a deletion in FRD3, a gene known to control iron-deficiency responses in A. thaliana. Based on the frequency of elemental profile mutations, we estimate 2-4% of the A. thaliana genome is involved in regulating the plant's nutrient and trace element content. These results demonstrate the utility of elemental profiling as a useful functional genomics tool.

AB - Understanding the functional connections between genes, proteins, metabolites and mineral ions is one of biology's greatest challenges in the postgenomic era. We describe here the use of mineral nutrient and trace element profiling as a tool to determine the biological significance of connections between a plant's genome and its elemental profile. Using inductively coupled plasma spectroscopy, we quantified 18 elements, including essential macro- and micronutrients and various nonessential elements, in shoots of 6,000 mutagenized M2 Arabidopsis thaliana plants. We isolated 51 mutants with altered elemental profiles. One mutant contains a deletion in FRD3, a gene known to control iron-deficiency responses in A. thaliana. Based on the frequency of elemental profile mutations, we estimate 2-4% of the A. thaliana genome is involved in regulating the plant's nutrient and trace element content. These results demonstrate the utility of elemental profiling as a useful functional genomics tool.

UR - http://www.scopus.com/inward/record.url?scp=10744223858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744223858&partnerID=8YFLogxK

U2 - 10.1038/nbt865

DO - 10.1038/nbt865

M3 - Article

C2 - 12949535

AN - SCOPUS:10744223858

VL - 21

SP - 1215

EP - 1221

JO - Biotechnology

JF - Biotechnology

SN - 1087-0156

IS - 10

ER -