Genomic features of first bovine astrovirus detected in Egypt

Shimaa M.G. Mansour, Fakry F. Mohamed, Fatma M. Abdallah, Iman E. El-Araby, Sunil K. Mor, Sagar M. Goyal

Research output: Contribution to journalArticlepeer-review

Abstract

Bovine astrovirus (BAstV) is a small single-stranded RNA virus, which belongs taxonomically to genus Mamastrovirus under the family Astroviridae. The BAstV is strongly linked to neonatal diarrhea of calves. A few studies are available on BAstV, mainly from Asia, and to a lesser extent from Europe, South America, and Africa. There is only one report from Egypt, in which BAstV was found in diarrheic calves, either in single- or co-infections, based on reverse transcription polymerase chain reaction (RT-PCR) and BAstV-polymerase enzyme targeting primers. One of the samples was further subjected to genomic characterization using Illumina platform for next generation sequencing (NGS). After being processed, the returned BAstV complete genome was subjected to sequence and phylogenetic analysis in comparison to reference strains. The BAstV open reading frames (ORF1a, ORF1b, and ORF2) followed a nearly similar genetic topology, as they belonged to the same unclassified lineage, which was earlier proposed as BAstV-lineage 1, and is known to be disseminated worldwide. This close phylogenetic relationship between the study strain and other members of this lineage was further confirmed by high nucleotide and amino acid (aa) identities. Additionally, a total of 24 unique aa residues were found through the entire BAstV genome. As being the first report in Egypt, indeed Africa, we believe that this record shall be useful in either taxonomic classification or epidemiological tracking of BAstV. The status of BAstV in Egypt should be carefully investigated with possible to-be-implemented precautions for the protection of animal-raising industries.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13337-021-00668-5.

Original languageEnglish (US)
Pages (from-to)601-607
Number of pages7
JournalVirusDisease
Volume32
Issue number3
DOIs
StatePublished - Sep 15 2021

Bibliographical note

Funding Information:
We thank Dr. Hany Abdalla (Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Egypt) for help in collection of samples and historical data. We are also grateful for the assistance of Todd P. Knutson (Supercomputing Institute for Advanced Computational Research, University of Minnesota) in the bioinformatics analysis.

Funding Information:
Fakry F. Mohamed received a visiting grant to the University of Minnesota from Ministry of Higher Education and Scientific Research (MHESR) in Egypt.

Publisher Copyright:
© 2021, Indian Virological Society.

Keywords

  • Calf diarrhea
  • Cattle
  • Mamastrovirus
  • Neonate
  • Phylogeny

PubMed: MeSH publication types

  • Journal Article

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