Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses

K. L. Beemon, A. J. Faras, A. T. Haase

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The genetic complexities of several ribodeoxyviruses were measured by quantitative analysis of unique RNase T1 resistant oligonucleotides from 60-70S viral RNAs. Moloney murine leukemia virus was found to have an RNA complexity of 3.5x106 daltons, whereas Moloney murine sarcoma virus had a significantly smaller genome size of 2.3x106. Reticuloendotheliosis and visna virus RNAs had complexities of 3.9x106 and 3.5x106, respectively. Analysis of RNase A resistant oligonucleotides of Rous sarcoma virus RNA gave a complexity of 3.6x106, similar to that previously obtained with RNase T1 resistant oligonucleotides. Since each of these viruses was found to have a unique sequence genomic complexity near the molecular weight of a single 30-40S viral RNA sub unit, it was concluded that ribodeoxyvirus genomes are at least largely polypoid.

Original languageEnglish (US)
Pages (from-to)525-537
Number of pages13
JournalJournal of Virology
Volume17
Issue number2
StatePublished - Dec 1 1976

Fingerprint

Reticuloendotheliosis virus
Visna-maedi virus
Murine Sarcoma Viruses
Visna maedi virus
Murine Leukemia Viruses
sarcoma
leukemia
Oligonucleotides
Ribonuclease T1
Viral RNA
RNA
genomics
oligonucleotides
mice
Moloney murine sarcoma virus
Moloney murine leukemia virus
Genome Size
Rous sarcoma virus
Pancreatic Ribonuclease
Murine leukemia virus

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Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses. / Beemon, K. L.; Faras, A. J.; Haase, A. T.

In: Journal of Virology, Vol. 17, No. 2, 01.12.1976, p. 525-537.

Research output: Contribution to journalArticle

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