TY - JOUR
T1 - Genomic characterization of a novel calicivirus, FHMCV-2012, from baitfish in the USA
AU - Mor, Sunil Kumar
AU - Phelps, Nicholas B.D.
AU - Ng, Terry Fei Fan
AU - Subramaniam, Kuttichantran
AU - Primus, Alexander
AU - Armien, Anibal G.
AU - McCann, Rebekah
AU - Puzach, Corey
AU - Waltzek, Thomas B.
AU - Goyal, Sagar M.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - During regulatory sampling of fathead minnows (Pimephales promelas), a novel calicivirus was isolated from homogenates of kidney and spleen inoculated into bluegill fry (BF-2) cells. Infected cell cultures exhibiting cytopathic effects were screened by PCR-based methods for selected fish viral pathogens. Illumina HiSeq next generation sequencing of the total RNA revealed a novel calicivirus genome that showed limited protein sequence similarity to known homologs in a BLASTp search. The complete genome of this fathead minnow calicivirus (FHMCV) is 6564 nt long, encoding a polyprotein of 2114 aa in length. The complete polyprotein shared only 21% identity with Atlantic salmon calicivirus,followed by 11% to 14% identity with mammalian caliciviruses. A molecular detection assay (RT-PCR) was designed from this sequence for screening of field samples for FHMCV in the future. This virus likely represents a prototype species of a novel genus in the family Caliciviridae, tentatively named “Minovirus”.
AB - During regulatory sampling of fathead minnows (Pimephales promelas), a novel calicivirus was isolated from homogenates of kidney and spleen inoculated into bluegill fry (BF-2) cells. Infected cell cultures exhibiting cytopathic effects were screened by PCR-based methods for selected fish viral pathogens. Illumina HiSeq next generation sequencing of the total RNA revealed a novel calicivirus genome that showed limited protein sequence similarity to known homologs in a BLASTp search. The complete genome of this fathead minnow calicivirus (FHMCV) is 6564 nt long, encoding a polyprotein of 2114 aa in length. The complete polyprotein shared only 21% identity with Atlantic salmon calicivirus,followed by 11% to 14% identity with mammalian caliciviruses. A molecular detection assay (RT-PCR) was designed from this sequence for screening of field samples for FHMCV in the future. This virus likely represents a prototype species of a novel genus in the family Caliciviridae, tentatively named “Minovirus”.
UR - http://www.scopus.com/inward/record.url?scp=85027504533&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027504533&partnerID=8YFLogxK
U2 - 10.1007/s00705-017-3519-6
DO - 10.1007/s00705-017-3519-6
M3 - Article
C2 - 28815386
AN - SCOPUS:85027504533
SN - 0304-8608
VL - 162
SP - 3619
EP - 3627
JO - Archives of Virology
JF - Archives of Virology
IS - 12
ER -