Genomic characterization of a novel calicivirus, FHMCV-2012, from baitfish in the USA

Sunil Kumar Mor, Nicholas B.D. Phelps, Terry Fei Fan Ng, Kuttichantran Subramaniam, Alexander Primus, Anibal G. Armien, Rebekah McCann, Corey Puzach, Thomas B. Waltzek, Sagar M. Goyal

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

During regulatory sampling of fathead minnows (Pimephales promelas), a novel calicivirus was isolated from homogenates of kidney and spleen inoculated into bluegill fry (BF-2) cells. Infected cell cultures exhibiting cytopathic effects were screened by PCR-based methods for selected fish viral pathogens. Illumina HiSeq next generation sequencing of the total RNA revealed a novel calicivirus genome that showed limited protein sequence similarity to known homologs in a BLASTp search. The complete genome of this fathead minnow calicivirus (FHMCV) is 6564 nt long, encoding a polyprotein of 2114 aa in length. The complete polyprotein shared only 21% identity with Atlantic salmon calicivirus,followed by 11% to 14% identity with mammalian caliciviruses. A molecular detection assay (RT-PCR) was designed from this sequence for screening of field samples for FHMCV in the future. This virus likely represents a prototype species of a novel genus in the family Caliciviridae, tentatively named “Minovirus”.

Original languageEnglish (US)
Pages (from-to)3619-3627
Number of pages9
JournalArchives of Virology
Volume162
Issue number12
DOIs
StatePublished - Dec 1 2017

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