Antisense technology has been widely used to regulate gene expression. A tetracycline (tet)-regulated antisense-RNA-expressing system has been developed and used to downregulate chromosomally derived genes expressed in Staphylococcus aureus. This downregulation subsequently provides an evaluation of the virulence factor and drug targets. The regulated antisense RNA library allows for genomewide analyses of the functions of staphylococcal gene products for growth in culture and survival during infection. Moreover, this antisense RNA technology may provide a key tool to identify mechanisms of novel antibacterial compound action.
Bibliographical noteFunding Information:
We thank Martin Rosenberg, David Holmes, David Payne and Martin Burnham for helpful discussions and suggestions. This work was supported in part by a research grant for the United States Defense Advanced Research Projects Agency (N65236-97-1-5810). The content of this publication does not necessary reflect the position or policy of the United States Government, and no official endorsement should be inferred.
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