TY - CHAP
T1 - Genomic analysis of gene expression of Staphylococcus aureus
AU - Yu, Chuanxin
AU - Sun, Junsong
AU - Zheng, Li
AU - Ji, Yinduo
PY - 2007/10/1
Y1 - 2007/10/1
N2 - The microarray has shown tremendous potential for investigating gene expression profiles and expression levels in comparative biology; exploring the regulation mechanisms of gene expression; and evaluating target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. In this chapter, we provide a detailed protocol for scientists who wish to investigate gene expression profiles by performing a microarray analysis, including different methods of RNA purification, decontamination, cDNA synthesis, fragmentation, and biotin labeling for hybridization using Affymetrix Staphylococcus aureus chips.
AB - The microarray has shown tremendous potential for investigating gene expression profiles and expression levels in comparative biology; exploring the regulation mechanisms of gene expression; and evaluating target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. In this chapter, we provide a detailed protocol for scientists who wish to investigate gene expression profiles by performing a microarray analysis, including different methods of RNA purification, decontamination, cDNA synthesis, fragmentation, and biotin labeling for hybridization using Affymetrix Staphylococcus aureus chips.
KW - Gene expression
KW - Microarray analysis
KW - Real-time reverse transcription polymerase chain reaction (RT-PCR)
UR - http://www.scopus.com/inward/record.url?scp=36549014039&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36549014039&partnerID=8YFLogxK
U2 - 10.1385/1-59745-468-0:169
DO - 10.1385/1-59745-468-0:169
M3 - Chapter
C2 - 18025677
AN - SCOPUS:36549014039
SN - 1597454680
SN - 9781597454681
T3 - Methods in Molecular Biology
SP - 169
EP - 178
BT - Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols
A2 - Ji, Yinduo
ER -
