Genomic analysis of gene expression of Staphylococcus aureus

Chuanxin Yu, Junsong Sun, Li Zheng, Yinduo Ji

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


The microarray has shown tremendous potential for investigating gene expression profiles and expression levels in comparative biology; exploring the regulation mechanisms of gene expression; and evaluating target gene for developing new chemotherapeutic agents, vaccine, and diagnostic methods. In this chapter, we provide a detailed protocol for scientists who wish to investigate gene expression profiles by performing a microarray analysis, including different methods of RNA purification, decontamination, cDNA synthesis, fragmentation, and biotin labeling for hybridization using Affymetrix Staphylococcus aureus chips.

Original languageEnglish (US)
Title of host publicationMethicillin-Resistant Staphylococcus aureus (MRSA) Protocols
EditorsYinduo Ji
Number of pages10
StatePublished - Oct 1 2007

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Gene expression
  • Microarray analysis
  • Real-time reverse transcription polymerase chain reaction (RT-PCR)


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