Genome-wide metabolic network reconstruction of the picoalga Ostreococcus

Elias W. Krumholz, Hong Yang, Pamela Weisenhorn, Christopher S. Henry, Igor G L Libourel

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The green picoalga Ostreococcus is emerging as a simple plant model organism, and two species, O. lucimarinus and O. tauri, have now been sequenced and annotated manually. To evaluate the completeness of the metabolic annotation of both species, metabolic networks of O. lucimarinus and O. tauri were reconstructed from the KEGG database, thermodynamically constrained, elementally balanced, and functionally evaluated. The draft networks contained extensive gaps and, in the case of O. tauri, no biomass components could be produced due to an incomplete Calvin cycle. To find and remove gaps from the networks, an extensive reference biochemical reaction database was assembled using a stepwise approach that minimized the inclusion of microbial reactions. Gaps were then removed from both Ostreococcus networks using two existing gap-filling methodologies. In the first method, a bottom-up approach, a minimal list of reactions was added to each model to enable the production of all metabolites included in our biomass equation. In the second method, a top-down approach, all reactions in the reference database were added to the target networks and subsequently trimmed away based on the sequence alignment scores of identified orthologues. Because current gap-filling methods do not produce unique solutions, a quality metric that includes a weighting for phylogenetic distance and sequence similarity was developed to distinguish between gap-filling results automatically. The draft O. lucimarinus and O. tauri networks required the addition of 56 and 70 reactions, respectively, in order to produce the same biomass precursor metabolites that were produced by our plant reference database.

Original languageEnglish (US)
Pages (from-to)2353-2362
Number of pages10
JournalJournal of experimental botany
Volume63
Issue number6
DOIs
StatePublished - Mar 2012

Bibliographical note

Funding Information:
The authors wish to express their gratitude to George Weiblen (university of Minnesota) for supplying the phylogenetic relationship between plant clades and aiding in the interpretation of the phylogenetic distance estimates. We are grateful for the assistance from Zhengjin Tu for setting up high throughput sequence comparison, and the Minnesota Supercomputing Institute for providing the computational infrastructure to make this work possible. Above all, we value the help we received from Ron Milo, Avi Flamholz, and Elad Noor (Weizmann Institute) in determining the flux directionality based on group contribution calculations. HY and IGLL are supported by NSF MCB award 1042335.

Keywords

  • Gap-filling
  • Ostreococcus
  • metabolic network
  • network reconstruction

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