Genome-wide association studies with proteomics data reveal genes important for synthesis, transport and packaging of globulins in legume seeds

Christine Le Signor, Delphine Aimé, Amandine Bordat, Maya Belghazi, Valérie Labas, Jérôme Gouzy, Nevin D. Young, Jean Marie Prosperi, Olivier Leprince, Richard D. Thompson, Julia Buitink, Judith Burstin, Karine Gallardo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Improving nutritional seed quality is an important challenge in grain legume breeding. However, the genes controlling the differential accumulation of globulins, which are major contributors to seed nutritional value in legumes, remain largely unknown. We combined a search for protein quantity loci with genome-wide association studies on the abundance of 7S and 11S globulins in seeds of the model legume species Medicago truncatula. Identified genomic regions and genes carrying polymorphisms linked to globulin variations were then cross-compared with pea (Pisum sativum), leading to the identification of candidate genes for the regulation of globulin abundance in this crop. Key candidates identified include genes involved in transcription, chromatin remodeling, post-translational modifications, transport and targeting of proteins to storage vacuoles. Inference of a gene coexpression network of 12 candidate transcription factors and globulin genes revealed the transcription factor ABA-insensitive 5 (ABI5) as a highly connected hub. Characterization of loss-of-function abi5 mutants in pea uncovered a role for ABI5 in controlling the relative abundance of vicilin, a sulfur-poor 7S globulin, in pea seeds. This demonstrates the feasibility of using genome-wide association studies in M. truncatula to reveal genes that can be modulated to improve seed nutritional value.

Original languageEnglish (US)
Pages (from-to)1597-1613
Number of pages17
JournalNew Phytologist
Volume214
Issue number4
DOIs
StatePublished - Jun 1 2017

Fingerprint

Genome-Wide Association Study
Globulins
Product Packaging
globulins
Fabaceae
Proteomics
proteomics
packaging
Seeds
legumes
Peas
synthesis
seeds
Genes
peas
genes
Nutritive Value
Transcription Factors
transcription factors
nutritive value

Keywords

  • Medicago truncatula
  • Pisum sativum (pea)
  • genome-wide association studies (GWAS)
  • globulins
  • legumes
  • protein quantity loci (PQL)
  • seed proteome

PubMed: MeSH publication types

  • Journal Article

Cite this

Genome-wide association studies with proteomics data reveal genes important for synthesis, transport and packaging of globulins in legume seeds. / Le Signor, Christine; Aimé, Delphine; Bordat, Amandine; Belghazi, Maya; Labas, Valérie; Gouzy, Jérôme; Young, Nevin D.; Prosperi, Jean Marie; Leprince, Olivier; Thompson, Richard D.; Buitink, Julia; Burstin, Judith; Gallardo, Karine.

In: New Phytologist, Vol. 214, No. 4, 01.06.2017, p. 1597-1613.

Research output: Contribution to journalArticle

Le Signor, C, Aimé, D, Bordat, A, Belghazi, M, Labas, V, Gouzy, J, Young, ND, Prosperi, JM, Leprince, O, Thompson, RD, Buitink, J, Burstin, J & Gallardo, K 2017, 'Genome-wide association studies with proteomics data reveal genes important for synthesis, transport and packaging of globulins in legume seeds', New Phytologist, vol. 214, no. 4, pp. 1597-1613. https://doi.org/10.1111/nph.14500
Le Signor, Christine ; Aimé, Delphine ; Bordat, Amandine ; Belghazi, Maya ; Labas, Valérie ; Gouzy, Jérôme ; Young, Nevin D. ; Prosperi, Jean Marie ; Leprince, Olivier ; Thompson, Richard D. ; Buitink, Julia ; Burstin, Judith ; Gallardo, Karine. / Genome-wide association studies with proteomics data reveal genes important for synthesis, transport and packaging of globulins in legume seeds. In: New Phytologist. 2017 ; Vol. 214, No. 4. pp. 1597-1613.
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