Genome-wide association analysis of the strength of the MAMP-elicited defense response and resistance to target leaf spot in sorghum

Rozalynne Samira; Jennifer A. Kimball; Luis Fernando Samayoa; James B. Holland; Tiffany M. Jamann; Patrick J. Brown; Gary Stacey; Peter J. Balint-Kurti

doi:10.1038/s41598-020-77684-w

Genome-wide association analysis of the strength of the MAMP-elicited defense response and resistance to target leaf spot in sorghum

Rozalynne Samira, Jennifer A. Kimball, Luis Fernando Samayoa, James B. Holland, Tiffany M. Jamann, Patrick J. Brown, Gary Stacey, Peter J. Balint-Kurti

Agronomy and Plant Genetics

Research output: Contribution to journal › Article › peer-review

Abstract

Plants have the capacity to respond to conserved molecular features known as microbe-associated molecular patterns (MAMPs). The goal of this work was to assess variation in the MAMP response in sorghum, to map loci associated with this variation, and to investigate possible connections with variation in quantitative disease resistance. Using an assay that measures the production of reactive oxygen species, we assessed variation in the MAMP response in a sorghum association mapping population known as the sorghum conversion population (SCP). We identified consistent variation for the response to chitin and flg22—an epitope of flagellin. We identified two SNP loci associated with variation in the flg22 response and one with the chitin response. We also assessed resistance to Target Leaf Spot (TLS) disease caused by the necrotrophic fungus Bipolaris cookei in the SCP. We identified one strong association on chromosome 5 near a previously characterized disease resistance gene. A moderately significant correlation was observed between stronger flg22 response and lower TLS resistance. Possible reasons for this are discussed.

Original language	English (US)
Article number	20817
Journal	Scientific reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-020-77684-w
State	Published - Dec 2020

Bibliographical note

Funding Information:
We thank Dr. Steve Kresovich for advice on seed handling and agronomic practice, and Dr. Burt Bluhm for providing isolates of B. cookei. We thank Cathy Herring and the staff at Central Crops Research Station for their work facilitating the field trials. Dr. Shannon Sermons and Greg Marshall assisted with several aspects of the field research. This work was funded by the DOE Plant Feedstock Genomics for Bioenergy program grants # DE-SC0014116 and DE-SC0019189.

PubMed: MeSH publication types

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

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Genome-wide association analysis of the strength of the MAMP-elicited defense response and resistance to target leaf spot in sorghum. / Samira, Rozalynne; Kimball, Jennifer A.; Samayoa, Luis Fernando; Holland, James B.; Jamann, Tiffany M.; Brown, Patrick J.; Stacey, Gary; Balint-Kurti, Peter J.

In: Scientific reports, Vol. 10, No. 1, 20817, 12.2020.

Research output: Contribution to journal › Article › peer-review

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abstract = "Plants have the capacity to respond to conserved molecular features known as microbe-associated molecular patterns (MAMPs). The goal of this work was to assess variation in the MAMP response in sorghum, to map loci associated with this variation, and to investigate possible connections with variation in quantitative disease resistance. Using an assay that measures the production of reactive oxygen species, we assessed variation in the MAMP response in a sorghum association mapping population known as the sorghum conversion population (SCP). We identified consistent variation for the response to chitin and flg22—an epitope of flagellin. We identified two SNP loci associated with variation in the flg22 response and one with the chitin response. We also assessed resistance to Target Leaf Spot (TLS) disease caused by the necrotrophic fungus Bipolaris cookei in the SCP. We identified one strong association on chromosome 5 near a previously characterized disease resistance gene. A moderately significant correlation was observed between stronger flg22 response and lower TLS resistance. Possible reasons for this are discussed.",

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