Genome sequences and annotation of two urinary isolates of E. coli

Travis K. Price; Arya Mehrtash; Laurynas Kalesinskas; Kema Malki; Evann E. Hilt; Catherine Putonti; Alan J. Wolfe

doi:10.1186/s40793-016-0202-6

Genome sequences and annotation of two urinary isolates of E. coli

Travis K. Price, Arya Mehrtash, Laurynas Kalesinskas, Kema Malki, Evann E. Hilt, Catherine Putonti, Alan J. Wolfe

Research output: Contribution to journal › Article › peer-review

Abstract

The genus Escherichia includes pathogens and commensals. Bladder infections (cystitis) result most often from colonization of the bladder by uropathogenic E. coli strains. In contrast, a poorly defined condition called asymptomatic bacteriuria results from colonization of the bladder with E. coli strains without symptoms. As part of an on-going attempt to identify and characterize the newly discovered female urinary microbiota, we report the genome sequences and annotation of two urinary isolates of E. coli: one (E78) was isolated from a female patient who self-reported cystitis; the other (E75) was isolated from a female patient who reported that she did not have symptoms of cystitis. Whereas strain E75 is most closely related to an avian extraintestinal pathogen, strain E78 is a member of a clade that includes extraintestinal strains often found in the human bladder. Both genomes are uncommonly rich in prophages.

Original language	English (US)
Article number	79
Journal	Standards in Genomic Sciences
Volume	11
Issue number	1
DOIs	https://doi.org/10.1186/s40793-016-0202-6
State	Published - Oct 12 2016
Externally published	Yes

Bibliographical note

Funding Information:
We would like to acknowledge Female Pelvic Medicine & Reconstructive Surgery at Loyola University Medical Center for their help in the patient recruitment and sample collection that led to isolation of these strains, Dr. Doerte Lehmann for aiding in the TEM sample preparation protocol, and Linda Fox from the Core Imaging Facility at Loyola University Chicago for obtaining the TEM images. We also acknowledge Gina Kuffel and Dr. Michael Zilliox for sequencing the genomes. AJW and CP are supported by Loyola University Chicago’s Multidisciplinary Research Award. This work was also partially funded by the NSF (1149387 to CP) and the NIH (R21DK097435-01A1 to AJW).

Publisher Copyright:
© 2016 The Author(s).

Keywords

Bladder
Enterobacteriaceae
Escherichia coli
Lower urinary tract symptoms
UPEC
Urinary tract infection

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abstract = "The genus Escherichia includes pathogens and commensals. Bladder infections (cystitis) result most often from colonization of the bladder by uropathogenic E. coli strains. In contrast, a poorly defined condition called asymptomatic bacteriuria results from colonization of the bladder with E. coli strains without symptoms. As part of an on-going attempt to identify and characterize the newly discovered female urinary microbiota, we report the genome sequences and annotation of two urinary isolates of E. coli: one (E78) was isolated from a female patient who self-reported cystitis; the other (E75) was isolated from a female patient who reported that she did not have symptoms of cystitis. Whereas strain E75 is most closely related to an avian extraintestinal pathogen, strain E78 is a member of a clade that includes extraintestinal strains often found in the human bladder. Both genomes are uncommonly rich in prophages.",

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note = "Funding Information: We would like to acknowledge Female Pelvic Medicine & Reconstructive Surgery at Loyola University Medical Center for their help in the patient recruitment and sample collection that led to isolation of these strains, Dr. Doerte Lehmann for aiding in the TEM sample preparation protocol, and Linda Fox from the Core Imaging Facility at Loyola University Chicago for obtaining the TEM images. We also acknowledge Gina Kuffel and Dr. Michael Zilliox for sequencing the genomes. AJW and CP are supported by Loyola University Chicago{\textquoteright}s Multidisciplinary Research Award. This work was also partially funded by the NSF (1149387 to CP) and the NIH (R21DK097435-01A1 to AJW). Publisher Copyright: {\textcopyright} 2016 The Author(s).",

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N2 - The genus Escherichia includes pathogens and commensals. Bladder infections (cystitis) result most often from colonization of the bladder by uropathogenic E. coli strains. In contrast, a poorly defined condition called asymptomatic bacteriuria results from colonization of the bladder with E. coli strains without symptoms. As part of an on-going attempt to identify and characterize the newly discovered female urinary microbiota, we report the genome sequences and annotation of two urinary isolates of E. coli: one (E78) was isolated from a female patient who self-reported cystitis; the other (E75) was isolated from a female patient who reported that she did not have symptoms of cystitis. Whereas strain E75 is most closely related to an avian extraintestinal pathogen, strain E78 is a member of a clade that includes extraintestinal strains often found in the human bladder. Both genomes are uncommonly rich in prophages.

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Genome sequences and annotation of two urinary isolates of E. coli

Abstract

Bibliographical note

Keywords

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