Genome sequence of human cytomegalovirus Ig-KG-H2, a variant of strain KG propagated in the presence of neutralizing antibodies

Ahmed Al Qaffas; Salvatore Camiolo; Jenna Nichols; Andrew J. Davison; Amine Ourahmane; Xiaohong Cui; Mark R. Schleiss; Laura Hertel; Dirk P. Dittmer; Michael A. McVoy

doi:10.1128/MRA.00063-20

Genome sequence of human cytomegalovirus Ig-KG-H2, a variant of strain KG propagated in the presence of neutralizing antibodies

Ahmed Al Qaffas, Salvatore Camiolo, Jenna Nichols, Andrew J. Davison, Amine Ourahmane, Xiaohong Cui, Mark R. Schleiss, Laura Hertel, Dirk P. Dittmer, Michael A. McVoy

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Abstract

Human cytomegalovirus shed in infant urine was isolated and serially passaged in fibroblasts in the presence or absence of neutralizing antibodies. Comparison of the genome sequences of representative viruses Ig-KG-H2 (passed with antibody) and ϕ-KG-B5 (passed without antibody) revealed the presence of several mutations in each virus.

Original language	English (US)
Article number	e00063-20
Journal	Microbiology Resource Announcements
Volume	9
Issue number	17
DOIs	https://doi.org/10.1128/MRA.00063-20
State	Published - Apr 23 2020

Bibliographical note

Funding Information:
This work was supported by grants R01AI088750 and R21AI073615 (National Institutes of Health; to M.A.M.), R01AI128912 (National Institutes of Health; to M.A.M. and L.H.), R01HD079918 (National Institutes of Health; to M.R.S.), P01CA019014 (National Institutes of Health; to D.P.D.), 6-FY17-849 (March of Dimes Birth Defects Foundation; to

Funding Information:
We thank CSL Behring (King of Prussia, PA, USA) for the kind gift of CytoGam. This work was supported by grants R01AI088750 and R21AI073615 (National Institutes of Health; to M.A.M.), R01AI128912 (National Institutes of Health; to M.A.M. and L.H.), R01HD079918 (National Institutes of Health; to M.R.S.), P01CA019014 (National Institutes of Health; to D.P.D.), 6-FY17-849 (March of Dimes Birth Defects Foundation; to M.R.S.), 204870/Z/16/Z (Wellcome; to A.J.D.), MC_UU_12014/3 (Medical Research Council; to A.J.D.), and LKR141973 and LKRD119165 (Merck & Co.; to M.A.M.).

Publisher Copyright:
Copyright © 2020 Al Qaffas et al.

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Al Qaffas, A., Camiolo, S., Nichols, J., Davison, A. J., Ourahmane, A., Cui, X., Schleiss, M. R., Hertel, L., Dittmer, D. P., & McVoy, M. A. (2020). Genome sequence of human cytomegalovirus Ig-KG-H2, a variant of strain KG propagated in the presence of neutralizing antibodies. Microbiology Resource Announcements, 9(17), Article e00063-20. https://doi.org/10.1128/MRA.00063-20

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abstract = "Human cytomegalovirus shed in infant urine was isolated and serially passaged in fibroblasts in the presence or absence of neutralizing antibodies. Comparison of the genome sequences of representative viruses Ig-KG-H2 (passed with antibody) and ϕ-KG-B5 (passed without antibody) revealed the presence of several mutations in each virus.",

author = "{Al Qaffas}, Ahmed and Salvatore Camiolo and Jenna Nichols and Davison, {Andrew J.} and Amine Ourahmane and Xiaohong Cui and Schleiss, {Mark R.} and Laura Hertel and Dittmer, {Dirk P.} and McVoy, {Michael A.}",

note = "Funding Information: This work was supported by grants R01AI088750 and R21AI073615 (National Institutes of Health; to M.A.M.), R01AI128912 (National Institutes of Health; to M.A.M. and L.H.), R01HD079918 (National Institutes of Health; to M.R.S.), P01CA019014 (National Institutes of Health; to D.P.D.), 6-FY17-849 (March of Dimes Birth Defects Foundation; to Funding Information: We thank CSL Behring (King of Prussia, PA, USA) for the kind gift of CytoGam. This work was supported by grants R01AI088750 and R21AI073615 (National Institutes of Health; to M.A.M.), R01AI128912 (National Institutes of Health; to M.A.M. and L.H.), R01HD079918 (National Institutes of Health; to M.R.S.), P01CA019014 (National Institutes of Health; to D.P.D.), 6-FY17-849 (March of Dimes Birth Defects Foundation; to M.R.S.), 204870/Z/16/Z (Wellcome; to A.J.D.), MC_UU_12014/3 (Medical Research Council; to A.J.D.), and LKR141973 and LKRD119165 (Merck & Co.; to M.A.M.). Publisher Copyright: Copyright {\textcopyright} 2020 Al Qaffas et al.",

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AU - Davison, Andrew J.

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AU - Schleiss, Mark R.

AU - Hertel, Laura

AU - Dittmer, Dirk P.

AU - McVoy, Michael A.

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Genome sequence of human cytomegalovirus Ig-KG-H2, a variant of strain KG propagated in the presence of neutralizing antibodies

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