Genome scale mutational analysis of Geobacter sulfurreducens reveals distinct molecular mechanisms for respiration and sensing of poised electrodes versus Fe(III) oxides

Chi Ho Chan, Caleb E. Levar, Fernanda Jiménez-Otero, Daniel R. Bond

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25 Scopus citations

Abstract

Geobacter sulfurreducens generates electrical current by coupling intracellular oxidation of organic acids to the reduction of proteins on the cell surface that are able to interface with electrodes. This ability is attributed to the bacterium's capacity to respire other extracellular electron acceptors that require contact, such as insoluble metal oxides. To directly investigate the genetic basis of electrode-based respiration, we constructed Geobacter sulfurreducens transposon-insertion sequencing (Tn-Seq) libraries for growth, with soluble fumarate or an electrode as the electron acceptor. Libraries with >33,000 unique insertions and an average of 9 insertions/kb allowed an assessment of each gene's fitness in a single experiment. Mutations in 1,214 different genomic features impaired growth with fumarate, and the significance of 270 genes unresolved by annotation due to the presence of one or more functional homologs was determined. Tn-Seq analysis of -0.1 V versus standard hydrogen electrode (SHE) electrode-grown cells identified mutations in a subset of genes encoding cytochromes, processing systems for proline-rich proteins, sensory networks, extracellular structures, polysaccharides, and metabolic enzymes that caused at least a 50% reduction in apparent growth rate. Scarless deletion mutants of select genes identified via Tn-Seq revealed a new putative porin-cytochrome conduit complex (extABCD) crucial for growth with electrodes, which was not required for Fe(III) oxide reduction. In addition, four mutants lacking components of a putative methyl-accepting chemotaxis-cyclic dinucleotide sensing network (esnABCD) were defective in electrode colonization but grew normally with Fe(III) oxides. These results suggest that G. sulfurreducens possesses distinct mechanisms for recognition, colonization, and reduction of electrodes compared to Fe(III) oxides.

Original languageEnglish (US)
Article numbere00340-17
JournalJournal of bacteriology
Volume199
Issue number19
DOIs
StatePublished - 2017

Bibliographical note

Funding Information:
This study was supported by grants N000141210308 and N000141612194 from the Office of Naval Research. F. Jiménez-Otero was supported by the Mexican National Council for Science and Technology. C. E. Levar was supported by the State of Minnesota-University of Minnesota MNDrive program.

Publisher Copyright:
© 2017 American Society for Microbiology.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Extracellular electron transfer
  • Extracellular respiration
  • Geobacter
  • Multiheme cytochrome
  • Tn-Seq

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