Genome editing for crop improvement – applications in clonally propagated polyploids with a focus on potato (solanum tuberosum L.)

Satya Swathi Nadakuduti, C. Robin Buell, Daniel F. Voytas, Colby G. Starker, David S. Douches

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


Genome-editing has revolutionized biology. When coupled with a recently streamlined regulatory process by the U.S. Department of Agriculture and the potential to generate transgene-free varieties, genome-editing provides a new avenue for crop improvement. For heterozygous, polyploid and vegetatively propagated crops such as cultivated potato, Solanum tuberosum Group Tuberosum L., genome-editing presents tremendous opportunities for trait improvement. In potato, traits such as improved resistance to cold-induced sweetening, processing efficiency, herbicide tolerance, modified starch quality and self-incompatibility have been targeted utilizing CRISPR/Cas9 and TALEN reagents in diploid and tetraploid clones. However, limited progress has been made in other such crops including sweetpotato, strawberry, grapes, citrus, banana etc., In this review we summarize the developments in genome-editing platforms, delivery mechanisms applicable to plants and then discuss the recent developments in regulation of genome-edited crops in the United States and The European Union. Next, we provide insight into the challenges of genome-editing in clonally propagated polyploid crops, their current status for trait improvement with future prospects focused on potato, a global food security crop.

Original languageEnglish (US)
Article number1607
JournalFrontiers in Plant Science
StatePublished - 2018

Bibliographical note

Funding Information:
Funding for this study was provided by the Biotechnology Risk Assessment Grant Program competitive grant no. 2013-33522-21090 from the USDA National Institute of Food and Agriculture and the Agricultural Research Service.

Publisher Copyright:
© 2018 Nadakuduti, Buell, Voytas, Starker and Douches.


  • Agrobacterium-mediated transformation
  • CRISPR/Cas system
  • Clonal propagation
  • Genome-editing
  • Polyploidy
  • Potato (Solanum tuberosum)
  • Protoplast transformation
  • TALENs


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