Genome Characterization and Pathogenicity of Two New Hyptis pectinata Viruses Transmitted by Distinct Insect Vectors

Edison Reyes-Proaño, Robert Alvarez-Quinto, José A. Delgado-Jiménez, Juan F. Cornejo-Franco, Dimitre Mollov, Nicolás Bejerman, Diego F. Quito-Avila

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Two newly described viruses belonging to distinct families, Rhabdoviridae and Geminiviridae, were discovered co-infecting Hyptis pectinata from a tropical dry forest of Ecuador. The negative-sense RNA genome of the rhabdovirus, tentatively named Hyptis latent virus (HpLV), comprises 13,765 nucleotides with seven open reading frames separated by the conserved intergenic region 3'-AAUUAUUUUGAU-5'. Sequence analyses showed identities as high as 56% for the polymerase and 38% for the nucleocapsid to members of the genus Cytorhabdovirus. Efficient transmission of HpLV was mediated by the pea aphid (Acyrthosiphon pisum) in a persistent replicative manner. The single-stranded DNA genome of the virus tentatively named Hyptis golden mosaic virus (HpGMV) shared homology with members of the genus Begomovirus with bipartite genomes. The DNA-A component consists of 2,716 nucleotides (nt), whereas the DNA-B component contains 2,666 nt. Pairwise alignments using the complete genomic sequence of DNA-A of HpGMV and closest relatives showed identities below the cutoff (<91% shared nt) established by the ICTV as species demarcation, indicating that HpGMV should be classified in a distinct begomovirus species. Transmission experiments confirmed that the whitefly Bemisia tabaci Middle East-Asia Minor 1 (MEAM1) is a vector of HpGMV.

Original languageEnglish (US)
Pages (from-to)2440-2448
Number of pages9
JournalPhytopathology
Volume112
Issue number11
DOIs
StatePublished - Nov 1 2022
Externally publishedYes

Keywords

  • aphid transmission
  • begomovirus
  • cytorhabdovirus
  • weed
  • whitefly transmission

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