Genipin-crosslinked gelatin–silk fibroin hydrogels for modulating the behaviour of pluripotent cells

Wei Sun, Tania Incitti, Claudio Migliaresi, Alessandro Quattrone, Simona Casarosa, Antonella Motta

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Different hydrogel materials have been prepared to investigate the effects of culture substrate on the behaviour of pluripotent cells. In particular, genipin-crosslinked gelatin–silk fibroin hydrogels of different compositions have been prepared, physically characterized and used as substrates for the culture of pluripotent cells. Pluripotent cells cultured on hydrogels remained viable and proliferated. Gelatin and silk fibroin promoted the proliferation of cells in the short and long term, respectively. Moreover, cells cultured on genipin-crosslinked gelatin–silk fibroin blended hydrogels were induced to an epithelial ectodermal differentiation fate, instead of the neural ectodermal fate obtained by culturing on tissue culture plates. This work confirms that specific culture substrates can be used to modulate the behaviour of pluripotent cells and that our genipin-crosslinked gelatin–silk fibroin blended hydrogels can induce pluripotent cells differentiation to an epithelial ectodermal fate.

Original languageEnglish (US)
Pages (from-to)876-887
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume10
Issue number10
DOIs
StatePublished - Oct 1 2016

Keywords

  • differentiation
  • gelatin
  • genipin
  • hydrogel scaffold
  • pluripotent cells
  • silk fibroin

Fingerprint Dive into the research topics of 'Genipin-crosslinked gelatin–silk fibroin hydrogels for modulating the behaviour of pluripotent cells'. Together they form a unique fingerprint.

Cite this