Genetics and molecular mapping of resistance to necrosis inducing race 5 of Pyrenophora tritici-repentis in tetraploid wheat

P. K. Singh, M. Mergoum, J. L. Gonzalez-Hernandez, S. Ali, T. B. Adhikari, S. F. Kianian, E. M. Elias, G. R. Hughes

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Race 5 of Pyrenophora tritici-repentis, causal agent of tan spot, induces two distinct symptoms, necrosis and chlorosis in susceptible tetraploid and hexaploid wheat, respectively. This study was conducted under controlled environmental conditions to determine the inheritance of resistance to P. tritici-repentis, race 5, in a tetraploid wheat population and to map the resistance genes. Additionally, the relationship between the resistance genes effective against necrosis inducing races 3 and 5 in tetraploid wheat was determined. A population of 98 recombinant-inbred lines (RIL) was developed from a cross between the resistant genotype Triticum turgidum # 283 (PI352519) and the susceptible durum cultivar Coulter. This RIL population was screened individually with race 3 and race 5 and molecular mapping of the resistance gene(s) in this population was conducted. Additionally, the F2 and F4:5 generations of this population were screened with race 5 to determine the genetic control of resistance. Plants were inoculated at the two-leaf stage and disease reaction was assessed based on 1 to 5 lesion-type rating scale eight days after inoculation. Segregation analysis of the F 2 generation and of the F4:5 and F6:7 families indicated that a single recessive gene controlled resistance to necrosis induced by race 5. Analysis of the mapping data of the T. turgidum # 283/Coulter RIL population indicate that a major gene, designated tsn5, controlling resistance to race 5 is located on the long arm of chromosome 3B. The tsn5 gene is 8.3 cM proximal to the gene tsn2 that controls resistance to necrosis induced by race 3.

Original languageEnglish (US)
Pages (from-to)293-304
Number of pages12
JournalMolecular Breeding
Volume21
Issue number3
DOIs
StatePublished - Apr 2008

Bibliographical note

Funding Information:
Acknowledgements Financial support from Wheat Research and Promotion Council, Minnesota, USA, North Dakota Wheat Commission, and State Board of Agricultural Research and Education, North Dakota, USA is gratefully acknowledged. Technical help provided by Seema Singh during disease evaluation is greatly appreciated.

Keywords

  • Host resistance
  • Inheritance
  • Tan spot

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