Genetic transformation of the fungal plant wilt pathogen, Fusarium oxysporum

H. Corby Kistler, Ulla K. Benny

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Abstract

A system for transformation of the fungal plant pathogen Fusarium oxysporum has been developed. The system employs plasmids which contain a bacterial hygromycin B phosphotransferase gene (hph) linked to Aspergillus regulatory sequences and which confer hygromycin B resistance in Fusarium. Transformation resulted from integration of the vectors into heterologous regions of the Fusarium genome and occurred at a frequency of approximately one transformant per μg DNA. No evidence was found for autonomous replication of the vector in the fungus. The transformed, drug resistant phenotype was mitotically stable with or without selection. However, modification of integrated DNA could occur during vegetative growth.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalCurrent Genetics
Volume13
Issue number2
DOIs
StatePublished - Feb 1988

Keywords

  • Fungal transformation
  • Fusarium oxysporum
  • Plant pathogen

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