TY - JOUR
T1 - Genetic mapping of symbiotic loci on the Rhizobium meliloti chromosome.
AU - Glazebrook, J.
AU - Meiri, G.
AU - Walker, G. C.
N1 - Copyright:
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PY - 1992
Y1 - 1992
N2 - To facilitate genetic analyses of Rhizobium meliloti genes that are involved in symbiosis, we determined the map positions of 11 symbiotic loci on the R. meliloti chromosome by using a combination of the Tn5-Mob conjugational transfer method described by Klein et al. (S. Klein, K. Lohmann, G. C. Walker, and E. R. Signer. J. Bacteriol. 174:324-326, 1992) and co-transduction of genetic markers by bacteriophage phi M12. Loci involved in effective nodule formation (fix-379, fix-382, fix-383, fix-385, and fix-388), polysaccharide synthesis (exoR, exoS, exoC, and ndvB), nodule invasion (exoD), and nitrogen regulation (ntrA) were ordered with respect to previously mapped markers and each other. The positions of two other loci, degP and pho-1, were also determined.
AB - To facilitate genetic analyses of Rhizobium meliloti genes that are involved in symbiosis, we determined the map positions of 11 symbiotic loci on the R. meliloti chromosome by using a combination of the Tn5-Mob conjugational transfer method described by Klein et al. (S. Klein, K. Lohmann, G. C. Walker, and E. R. Signer. J. Bacteriol. 174:324-326, 1992) and co-transduction of genetic markers by bacteriophage phi M12. Loci involved in effective nodule formation (fix-379, fix-382, fix-383, fix-385, and fix-388), polysaccharide synthesis (exoR, exoS, exoC, and ndvB), nodule invasion (exoD), and nitrogen regulation (ntrA) were ordered with respect to previously mapped markers and each other. The positions of two other loci, degP and pho-1, were also determined.
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U2 - 10.1094/MPMI-5-223
DO - 10.1094/MPMI-5-223
M3 - Article
C2 - 1330089
AN - SCOPUS:0026867346
SN - 0894-0282
VL - 5
SP - 223
EP - 227
JO - Molecular plant-microbe interactions : MPMI
JF - Molecular plant-microbe interactions : MPMI
IS - 3
ER -