Genetic mapping of loci for resistance to stem rust in a tetraploid wheat collection

Antonietta Saccomanno, Oadi Matny, Daniela Marone, Giovanni Laidò, Giuseppe Petruzzino, Elisabetta Mazzucotelli, Francesca Desiderio, Antonio Blanco, Agata Gadaleta, Nicola Pecchioni, Pasquale De Vita, Brian Steffenson, Anna Maria Mastrangelo

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a major biotic constraint to wheat production worldwide. Disease resistant cultivars are a sustainable means for the efficient control of this disease. To identify quantitative trait loci (QTLs) conferring resistance to stem rust at the seedling stage, an association mapping panel consisting of 230 tetraploid wheat accessions were evaluated for reaction to five Pgt races under greenhouse conditions. A high level of phenotypic variation was observed in the panel in response to all of the races, allowing for genome-wide association mapping of resistance QTLs in wild, landrace, and cultivated tetraploid wheats. Twenty-two resistance QTLs were identified, which were characterized by at least two marker-trait associations. Most of the identified resistance loci were coincident with previously identified rust resistance genes/QTLs; however, six regions detected on chromosomes 1B, 5A, 5B, 6B, and 7B may be novel. Availability of the reference genome sequence of wild emmer wheat accession Zavitan facilitated the search for candidate resistance genes in the regions where QTLs were identified, and many of them were annotated as NOD (nucleotide binding oligomerization domain)-like receptor (NLR) genes or genes related to broad spectrum resistance.

Original languageEnglish (US)
Article number3907
JournalInternational journal of molecular sciences
Volume19
Issue number12
DOIs
StatePublished - Dec 2018

Bibliographical note

Funding Information:
Funding: This research was funded by the Italian Ministry of Foreign Affairs and International Cooperation, grant Res-Wheat (Boosting an healthier agriculture: identification of resistance genes for durum wheat cultivars more resistant to rust diseases), and by the University of Minnesota, grant number of 1802-11031-21374-0995029-AES00SG076 for Lieberman Okinow Endowment.

Funding Information:
This research was funded by the Italian Ministry of Foreign Affairs and International Cooperation, grant Res-Wheat (Boosting an healthier agriculture: identification of resistance genes for durum wheat cultivars more resistant to rust diseases), and by the University of Minnesota, grant number of 1802-11031-21374-0995029-AES00SG076 for Lieberman Okinow Endowment. Acknowledgments: We are grateful to the personnel of the USDA Cereal Disease Laboratory, Saint Paul, MN, for planting and inoculating the field test, and for the gift of the Pgt cultures.

Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Resistant loci
  • Stem rust
  • Tetraploid wheat

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