Genetic mapping and haplotype analysis of a locus for quantitative resistance to Fusarium graminearum in soybean accession PI 567516C

Peng Cheng, Cassidy R. Gedling, Gunvant Patil, Tri D. Vuong, J. Grover Shannon, Anne E. Dorrance, Henry T. Nguyen

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Key message: A major novel quantitative disease resistance locus, qRfg_Gm06, for Fusarium graminearum was genetically mapped to chromosome 6. Genomic-assisted haplotype analysis within this region identified three putative candidate genes. Abstract: Fusarium graminearum causes seed, root rot, and seedling damping-off in soybean which contributes to reduced stands and yield. A cultivar Magellan and PI 567516C were identified with low and high levels of partial resistance to F. graminearum, respectively. Quantitative disease resistance loci (QDRL) were mapped with 241 F7:8 recombinant inbred lines (RILs) derived from a cross of Magellan × PI 567516C. Phenotypic evaluation for resistance to F. graminearum used the rolled towel assay in a randomized incomplete block design. The genetic map was constructed from 927 polymorphic single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) markers. One major QDRL qRfg_Gm06 was detected and mapped to chromosome 6 with a LOD score of 20.3 explaining 40.2% of the total phenotypic variation. This QDRL was mapped to a ~400 kb genomic region of the Williams 82 reference genome. Genome mining of this region identified 14 putative candidate disease resistance genes. Haplotype analysis of this locus using whole genome re-sequencing (WGRS) of 106 diverse soybean lines narrowed the list to three genes. A SNP genotyping Kompetitive allele-specific PCR (KASP) assay was designed for one of the genes and was validated in a subset of the RILs and all 106 diverse lines.

Original languageEnglish (US)
Pages (from-to)999-1010
Number of pages12
JournalTheoretical and Applied Genetics
Issue number5
StatePublished - May 1 2017

Bibliographical note

Funding Information:
We thank Dr. H. Ye, D. J. Veney, M. Eyre, and L. Weber for technical assistance and T. Musket for editing of the manuscript. Funding for this project was provided by the United Soybean Board, the National Center for Soybean Biotechnology (University of Missouri), the Ohio Soybean Council, and the Ohio State University Center for Applied Plant Sciences. Salaries and research support for this project was provided by State and Federal Funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University and the National Institute of Food and Agriculture, U.S. Department of Agriculture, and Hatch project Development of Disease Management Strategies for Soybean Pathogens in Ohio OHO01303.

Funding Information:
Additional support was provided by the Ohio State University Center for Applied Plant Sciences as part of the Soybean Resistance Team project.

Publisher Copyright:
© 2017, Springer-Verlag Berlin Heidelberg.


Dive into the research topics of 'Genetic mapping and haplotype analysis of a locus for quantitative resistance to Fusarium graminearum in soybean accession PI 567516C'. Together they form a unique fingerprint.

Cite this