Genetic lineage tracing of non-cardiomyocytes in mice

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Genetic lineage tracing is accomplished using bi-transgenic mice, where one allele is altered to express Cre recombinase, and another allele encodes a Cre-dependent genetic reporter protein. Once Cre is activated (constitutive or in response to tamoxifen), the marker gene-expressing cells become indelibly labeled by the reporter protein. Therefore, daughter cells derived from labeled cells are permanently labeled even if the marker gene that drove Cre recombinase expression is no longer expressed in these cells. This system is commonly used to label putative progenitor cells and determine the fate of their progeny. Here, we describe the use of c-kit-based genetic lineage-tracing mouse line as an example and discuss caveats for performing these types of experiments.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages14
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2021.


  • Differentiation
  • Genetic lineage-tracing
  • Progenitor cell
  • Tamoxifen
  • Transgenic mice
  • Myocytes, Cardiac/chemistry
  • Gene Expression
  • Stem Cells/chemistry
  • Cell Lineage/genetics
  • Mice, Transgenic
  • Integrases/genetics
  • Animals
  • Proto-Oncogene Proteins c-kit/analysis
  • Green Fluorescent Proteins/genetics
  • Cell Tracking/methods
  • Models, Animal
  • Mice
  • Genes, Reporter
  • Tamoxifen/pharmacology
  • Genetic Linkage

PubMed: MeSH publication types

  • Research Support, Non-U.S. Gov't
  • Journal Article
  • Research Support, N.I.H., Extramural


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