Embryogenic and regenerable tissue cultures are widely utilized in plant transformation, clonal propagation, and biological research applications. Germplasm utilized in those applications are limited, however, due to genotype-dependent culture response. The goal of this study was to identify genomic regions controlling embryogenic and regenerable tissue culture response in the globally important crop, maize (Zea mays L.), toward the long-term objective of developing approaches for genotype-independent plant genetic engineering and clonal propagation systems. An inbred maize line, WCIC2, nearly-isogenic to reference inbred B73, was developed by phenotypic selection and molecular marker analysis. WCIC2 has over 50x increase in tissue culture response relative to the recurrent parent, B73. This line was used to genetically fine-map a region on chromosome 3 controlling embryogenic and regenerable tissue culture response to a 23.9 Mb region. WCIC2 and derivatives will be useful materials to enable maize research in a genetic background similar to B73, and our genetic mapping results will advance research to identify causal genes controlling somatic embryo formation and plant regeneration in maize.
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The authors of this paper would like to acknowledge the contributions from AgReliant Genetics for providing doubled haploid stocks in this study. We would also like to acknowledge the work conducted by Jill Mahoy as tissue culture lab manager who contributed to training personnel on tissue culture best practices. This study was based on work supported by the National Institute of Food and Agriculture, United States Department of Agriculture, Hatch project 0211033.
© Crop Science Society of America.