Genetic expression of endothelial nitric oxide synthase in human atrial myocardium

Chi Ming Wei, Shi Wen Jiang, John A. Lust, Richard C. Daly, Christopher G.A. Mcgregor

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Objective: To investigate the expression of endothelial nitric oxide synthase (eNOS) in human cardiac tissues. Design: We attempted to determine the genetic expression and localization of eNOS in normal human atrial tissue. Material and Methods: In normal human right atrial tissues from five donors during cardiac transplantation, eNOS expression and localization were assessed by using Northern blot analysis, in situ hybridization, and immunohistochemical staining. Results: Northern blot analysis and in situ hybridization demonstrated that eNOS messenger RNA is present in cardiomyocytes. Positive immunohistochemical staining was observed in the cytoplasm of cardiomyocytes. Conclusion: These studies show for the first time the genetic expression and distribution of eNOS in human atrial myocardium and suggest that the eNOS-mediated paracrine and autocrine pathway may participate in the control of myocardial function in humans.

Original languageEnglish (US)
Pages (from-to)346-350
Number of pages5
JournalMayo Clinic Proceedings
Volume71
Issue number4
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

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