Abstract
Objective: To investigate the expression of endothelial nitric oxide synthase (eNOS) in human cardiac tissues. Design: We attempted to determine the genetic expression and localization of eNOS in normal human atrial tissue. Material and Methods: In normal human right atrial tissues from five donors during cardiac transplantation, eNOS expression and localization were assessed by using Northern blot analysis, in situ hybridization, and immunohistochemical staining. Results: Northern blot analysis and in situ hybridization demonstrated that eNOS messenger RNA is present in cardiomyocytes. Positive immunohistochemical staining was observed in the cytoplasm of cardiomyocytes. Conclusion: These studies show for the first time the genetic expression and distribution of eNOS in human atrial myocardium and suggest that the eNOS-mediated paracrine and autocrine pathway may participate in the control of myocardial function in humans.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 346-350 |
| Number of pages | 5 |
| Journal | Mayo Clinic Proceedings |
| Volume | 71 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1996 |
| Externally published | Yes |
Bibliographical note
Funding Information:This study was supported in part by grants from the American Heart Association Minnesota Affiliate ( MN-123 ), Astra/Merck Group, American Society of Hypertension (MMD Clinical Fellowship), and National Institutes of Health, Public Health Service ( HL 03174-01 ).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
