Genetic expression of complement factors and α1-acid glycoprotein by liver tissue during senescence

Mark S. Rutherford; Carla S. Baehler; Arlan Richardson

doi:10.1016/0047-6374(86)90127-2

Genetic expression of complement factors and α₁-acid glycoprotein by liver tissue during senescence

Mark S. Rutherford, Carla S. Baehler, Arlan Richardson

Administration (CVM)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The effect of age on several messenger RNAs coding for non-specific immune factors were determined in liver RNA isolated from 6-, 12-, 24-, 29- and 37-month-old male Fischer Rats. The levels of complement factors C3 and C4, complement protein factor B, and α₁-acid glycoprotein were determined by dot blot hybridization using cDNA probes. All four mRNAs increased slightly between 6 and 29 months of age. Only the mRNAs coding for complement factors C3 and C4 decreased significantly after 29 months of age. In addition, Northern blot analysis of the RNA preparations showed that the size of the four mRNA species did not change with increasing age. There was no evidence for age-related changes in the post-transcriptional processing or degradation of the mRNA species coding for complement factors C3 and C4, complement protein factor B, and α₁-acid glycoprotein.

Original language	English (US)
Pages (from-to)	245-254
Number of pages	10
Journal	Mechanisms of Ageing and Development
Volume	35
Issue number	3
DOIs	https://doi.org/10.1016/0047-6374(86)90127-2
State	Published - Aug 1986

Keywords

Complement C3
Complement C4
Complement protein factor B
Messenger RNA
Transcription
α-Acid glycoprotein

Publisher link

10.1016/0047-6374(86)90127-2

Find It

Find It at U of M Libraries

Cite this

@article{def426c8951f4f518ee03fc7f414eafc,

title = "Genetic expression of complement factors and α1-acid glycoprotein by liver tissue during senescence",

abstract = "The effect of age on several messenger RNAs coding for non-specific immune factors were determined in liver RNA isolated from 6-, 12-, 24-, 29- and 37-month-old male Fischer Rats. The levels of complement factors C3 and C4, complement protein factor B, and α1-acid glycoprotein were determined by dot blot hybridization using cDNA probes. All four mRNAs increased slightly between 6 and 29 months of age. Only the mRNAs coding for complement factors C3 and C4 decreased significantly after 29 months of age. In addition, Northern blot analysis of the RNA preparations showed that the size of the four mRNA species did not change with increasing age. There was no evidence for age-related changes in the post-transcriptional processing or degradation of the mRNA species coding for complement factors C3 and C4, complement protein factor B, and α1-acid glycoprotein.",

keywords = "Complement C3, Complement C4, Complement protein factor B, Messenger RNA, Transcription, α-Acid glycoprotein",

author = "Rutherford, {Mark S.} and Baehler, {Carla S.} and Arlan Richardson",

year = "1986",

month = aug,

doi = "10.1016/0047-6374(86)90127-2",

language = "English (US)",

volume = "35",

pages = "245--254",

journal = "Mechanisms of Ageing and Development",

issn = "0047-6374",

publisher = "Elsevier Ireland Ltd",

number = "3",

}

TY - JOUR

T1 - Genetic expression of complement factors and α1-acid glycoprotein by liver tissue during senescence

AU - Rutherford, Mark S.

AU - Baehler, Carla S.

AU - Richardson, Arlan

PY - 1986/8

Y1 - 1986/8

N2 - The effect of age on several messenger RNAs coding for non-specific immune factors were determined in liver RNA isolated from 6-, 12-, 24-, 29- and 37-month-old male Fischer Rats. The levels of complement factors C3 and C4, complement protein factor B, and α1-acid glycoprotein were determined by dot blot hybridization using cDNA probes. All four mRNAs increased slightly between 6 and 29 months of age. Only the mRNAs coding for complement factors C3 and C4 decreased significantly after 29 months of age. In addition, Northern blot analysis of the RNA preparations showed that the size of the four mRNA species did not change with increasing age. There was no evidence for age-related changes in the post-transcriptional processing or degradation of the mRNA species coding for complement factors C3 and C4, complement protein factor B, and α1-acid glycoprotein.

AB - The effect of age on several messenger RNAs coding for non-specific immune factors were determined in liver RNA isolated from 6-, 12-, 24-, 29- and 37-month-old male Fischer Rats. The levels of complement factors C3 and C4, complement protein factor B, and α1-acid glycoprotein were determined by dot blot hybridization using cDNA probes. All four mRNAs increased slightly between 6 and 29 months of age. Only the mRNAs coding for complement factors C3 and C4 decreased significantly after 29 months of age. In addition, Northern blot analysis of the RNA preparations showed that the size of the four mRNA species did not change with increasing age. There was no evidence for age-related changes in the post-transcriptional processing or degradation of the mRNA species coding for complement factors C3 and C4, complement protein factor B, and α1-acid glycoprotein.

KW - Complement C3

KW - Complement C4

KW - Complement protein factor B

KW - Messenger RNA

KW - Transcription

KW - α-Acid glycoprotein

UR - http://www.scopus.com/inward/record.url?scp=0022919228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022919228&partnerID=8YFLogxK

U2 - 10.1016/0047-6374(86)90127-2

DO - 10.1016/0047-6374(86)90127-2

M3 - Article

C2 - 3640187

AN - SCOPUS:0022919228

SN - 0047-6374

VL - 35

SP - 245

EP - 254

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

IS - 3

ER -