Genetic diversity of the Chestnut blight fungus Cryphonectria parasitica in four French populations assessed by microsatellite markers

Florence Breuillin, Cyril Dutech, Cécile Robin

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41 Scopus citations

Abstract

Microsatellites are powerful markers to infer population genetic parameters. Here, 13 microsatellite loci isolated from a genomic and a cDNA library of Cryphonectria parasitica were used to characterize the genetic diversity and structure of four French populations. Twelve of these loci were polymorphic within populations, and average gene diversity (He) was estimated to be 0.35. There was a lower genetic diversity in a south-eastern population relative to three south-western populations. In these three populations, microsatellite genotypic diversity was higher than vegetative compatibility type diversity. A high genetic differentiation (GST = 0.27) suggested a low gene flow and/or founder effects of French populations which are in agreement with low dispersal of spores and different introductions of this species in southern France. This study demonstrates the significance of these microsatellite loci to assess gene flow and reproductive system in this important pathogen.

Original languageEnglish (US)
Pages (from-to)288-296
Number of pages9
JournalMycological Research
Volume110
Issue number3
DOIs
StatePublished - Mar 2006

Bibliographical note

Funding Information:
We thank Tatiana Giraud, Marie-Lance Desprez-Loustau, Michael Milgroom and three anonymous reviewers for helpful comments on the manuscript, and Françoise Delmotte for hosting F. B. in his laboratory (UMR Santé Végétale, INRA-Bordeaux) during this work. We also wish to thank Anne Giraud, Gilles Capron, Gaël Rolland and Xavier Capdevielle for help with the field collections; Henri Caron, Benoît Barrès, and Patrick Léger for their help in the genomic microsatellite development; David Chagné for the screening of the EST library; and Anne Giraud, Sylvie Richard-Cervera and Maïa Lafargue for help with the laboratory work. This work was supported by a grant from the Région Aquitaine.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

  • Founder effect
  • Introduced species
  • Invasive species
  • Plant pathology
  • Vegetative compatibility

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