Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat

C. D. Otto; S. F. Kianian; E. M. Elias; R. W. Stack; L. R. Joppa

doi:10.1023/A:1014821929830

Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat

C. D. Otto, S. F. Kianian, E. M. Elias, R. W. Stack, L. R. Joppa

Plant Pathology

Research output: Contribution to journal › Article › peer-review

105 Scopus citations

Abstract

The devastating effect of Fusarium head blight (FHB) caused by Fusarium graminearum has led to significant financial losses across the Upper Midwest of the USA. These losses have spurred the need for research in biological, chemical, and genetic control methods for this disease. To date, most of the research on FHB resistance has concentrated on hexaploid wheat (Triticum aestivum L.) lines originating from China. Other sources of resistance to FHB would be desirable. One other source of resistance for both hexaploid wheat and tetraploid durum wheat (T. turgidum L. var. durum) is the wild tetraploid, T. turgidum L. var. dicoccoides (T. dicoccoides). Previous analysis of the 'Langdon'-T. dicoccoides chromosome substitution lines, LDN(Dic), indicated that the chromosome 3A substitution line expresses moderate levels of resistance to FHB. LDN(Dic-3A) recombinant inbred chromosome lines (RICL) were used to generate a linkage map of chromosome 3A with 19 molecular markers spanning a distance of 155.2 cM. The individual RICL and controls were screened for their FHB phenotypein two greenhouse seasons. Analysis of 83 RICL identified a single major quantitative trait locus, Qfhs.ndsu-3AS, that explains 37% of the phenotypic or 55% of the genetic variation for FHB resistance. A microsatellite locus, Xgwm2, is tightly linked to the highest point of the QTL peak. A region of the LDN (Dic-3A) chromosome associated with the QTL for FHB resistance encompasses a 29.3 cM region from Xmwg14 to Xbcd828.

Original language	English (US)
Pages (from-to)	625-632
Number of pages	8
Journal	Plant molecular biology
Volume	48
Issue number	5-6
DOIs	https://doi.org/10.1023/A:1014821929830
State	Published - 2002

Bibliographical note

Funding Information:
We would like to thank Dr Jennifer Mitchell-Fetch, Mary Johnshoy, Dr. José González-Hernández, Jana Hansen, and Justin Hegstad for all their assistance in making this paper possible. Special thanks go to Erik Doehler for his work with the RGA primers. His help was made possible by a funding from the NSF-EPSCoR Science Bound Project. We would also like to thank the anonymous reviewers for their suggestions in improving this manuscript. We gratefully acknowledge grant support from the USDA-ARS, U.S. Wheat and Barley Scab Initiative (59-0790-9-048) and the North Dakota Wheat Commission to S.F.K.

Keywords

Durum wheat
Fusarium head blight
QTL
Recombinant inbred chromosome lines
Triticum dicoccoides

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1023/A:1014821929830

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@article{4b12c21e6a544fc8bf3580307bdde01e,

title = "Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat",

abstract = "The devastating effect of Fusarium head blight (FHB) caused by Fusarium graminearum has led to significant financial losses across the Upper Midwest of the USA. These losses have spurred the need for research in biological, chemical, and genetic control methods for this disease. To date, most of the research on FHB resistance has concentrated on hexaploid wheat (Triticum aestivum L.) lines originating from China. Other sources of resistance to FHB would be desirable. One other source of resistance for both hexaploid wheat and tetraploid durum wheat (T. turgidum L. var. durum) is the wild tetraploid, T. turgidum L. var. dicoccoides (T. dicoccoides). Previous analysis of the 'Langdon'-T. dicoccoides chromosome substitution lines, LDN(Dic), indicated that the chromosome 3A substitution line expresses moderate levels of resistance to FHB. LDN(Dic-3A) recombinant inbred chromosome lines (RICL) were used to generate a linkage map of chromosome 3A with 19 molecular markers spanning a distance of 155.2 cM. The individual RICL and controls were screened for their FHB phenotypein two greenhouse seasons. Analysis of 83 RICL identified a single major quantitative trait locus, Qfhs.ndsu-3AS, that explains 37% of the phenotypic or 55% of the genetic variation for FHB resistance. A microsatellite locus, Xgwm2, is tightly linked to the highest point of the QTL peak. A region of the LDN (Dic-3A) chromosome associated with the QTL for FHB resistance encompasses a 29.3 cM region from Xmwg14 to Xbcd828.",

keywords = "Durum wheat, Fusarium head blight, QTL, Recombinant inbred chromosome lines, Triticum dicoccoides",

author = "Otto, {C. D.} and Kianian, {S. F.} and Elias, {E. M.} and Stack, {R. W.} and Joppa, {L. R.}",

note = "Funding Information: We would like to thank Dr Jennifer Mitchell-Fetch, Mary Johnshoy, Dr. Jos{\'e} Gonz{\'a}lez-Hern{\'a}ndez, Jana Hansen, and Justin Hegstad for all their assistance in making this paper possible. Special thanks go to Erik Doehler for his work with the RGA primers. His help was made possible by a funding from the NSF-EPSCoR Science Bound Project. We would also like to thank the anonymous reviewers for their suggestions in improving this manuscript. We gratefully acknowledge grant support from the USDA-ARS, U.S. Wheat and Barley Scab Initiative (59-0790-9-048) and the North Dakota Wheat Commission to S.F.K.",

year = "2002",

doi = "10.1023/A:1014821929830",

language = "English (US)",

volume = "48",

pages = "625--632",

journal = "Plant molecular biology",

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publisher = "Springer Netherlands",

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AU - Otto, C. D.

AU - Kianian, S. F.

AU - Elias, E. M.

AU - Stack, R. W.

AU - Joppa, L. R.

N1 - Funding Information: We would like to thank Dr Jennifer Mitchell-Fetch, Mary Johnshoy, Dr. José González-Hernández, Jana Hansen, and Justin Hegstad for all their assistance in making this paper possible. Special thanks go to Erik Doehler for his work with the RGA primers. His help was made possible by a funding from the NSF-EPSCoR Science Bound Project. We would also like to thank the anonymous reviewers for their suggestions in improving this manuscript. We gratefully acknowledge grant support from the USDA-ARS, U.S. Wheat and Barley Scab Initiative (59-0790-9-048) and the North Dakota Wheat Commission to S.F.K.

PY - 2002

Y1 - 2002

N2 - The devastating effect of Fusarium head blight (FHB) caused by Fusarium graminearum has led to significant financial losses across the Upper Midwest of the USA. These losses have spurred the need for research in biological, chemical, and genetic control methods for this disease. To date, most of the research on FHB resistance has concentrated on hexaploid wheat (Triticum aestivum L.) lines originating from China. Other sources of resistance to FHB would be desirable. One other source of resistance for both hexaploid wheat and tetraploid durum wheat (T. turgidum L. var. durum) is the wild tetraploid, T. turgidum L. var. dicoccoides (T. dicoccoides). Previous analysis of the 'Langdon'-T. dicoccoides chromosome substitution lines, LDN(Dic), indicated that the chromosome 3A substitution line expresses moderate levels of resistance to FHB. LDN(Dic-3A) recombinant inbred chromosome lines (RICL) were used to generate a linkage map of chromosome 3A with 19 molecular markers spanning a distance of 155.2 cM. The individual RICL and controls were screened for their FHB phenotypein two greenhouse seasons. Analysis of 83 RICL identified a single major quantitative trait locus, Qfhs.ndsu-3AS, that explains 37% of the phenotypic or 55% of the genetic variation for FHB resistance. A microsatellite locus, Xgwm2, is tightly linked to the highest point of the QTL peak. A region of the LDN (Dic-3A) chromosome associated with the QTL for FHB resistance encompasses a 29.3 cM region from Xmwg14 to Xbcd828.

AB - The devastating effect of Fusarium head blight (FHB) caused by Fusarium graminearum has led to significant financial losses across the Upper Midwest of the USA. These losses have spurred the need for research in biological, chemical, and genetic control methods for this disease. To date, most of the research on FHB resistance has concentrated on hexaploid wheat (Triticum aestivum L.) lines originating from China. Other sources of resistance to FHB would be desirable. One other source of resistance for both hexaploid wheat and tetraploid durum wheat (T. turgidum L. var. durum) is the wild tetraploid, T. turgidum L. var. dicoccoides (T. dicoccoides). Previous analysis of the 'Langdon'-T. dicoccoides chromosome substitution lines, LDN(Dic), indicated that the chromosome 3A substitution line expresses moderate levels of resistance to FHB. LDN(Dic-3A) recombinant inbred chromosome lines (RICL) were used to generate a linkage map of chromosome 3A with 19 molecular markers spanning a distance of 155.2 cM. The individual RICL and controls were screened for their FHB phenotypein two greenhouse seasons. Analysis of 83 RICL identified a single major quantitative trait locus, Qfhs.ndsu-3AS, that explains 37% of the phenotypic or 55% of the genetic variation for FHB resistance. A microsatellite locus, Xgwm2, is tightly linked to the highest point of the QTL peak. A region of the LDN (Dic-3A) chromosome associated with the QTL for FHB resistance encompasses a 29.3 cM region from Xmwg14 to Xbcd828.

KW - Durum wheat

KW - Fusarium head blight

KW - QTL

KW - Recombinant inbred chromosome lines

KW - Triticum dicoccoides

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DO - 10.1023/A:1014821929830

M3 - Article

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AN - SCOPUS:0036010778

SN - 0167-4412

VL - 48

SP - 625

EP - 632

JO - Plant molecular biology

JF - Plant molecular biology

IS - 5-6

ER -

Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat

Abstract

Bibliographical note

Keywords

UN SDGs

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