Genetic control of morphometric diversity in the maize shoot apical meristem

Samuel Leiboff, Xianran Li, Heng Cheng Hu, Natalie Todt, Jinliang Yang, Xiao Li, Xiaoqing Yu, Gary J. Muehlbauer, Marja C.P. Timmermans, Jianming Yu, Patrick S. Schnable, Michael J. Scanlon

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38 Scopus citations

Abstract

The maize shoot apical meristem (SAM) comprises a small pool of stem cells that generate all above-ground organs. Although mutational studies have identified genetic networks regulating SAM function, little is known about SAM morphological variation in natural populations. Here we report the use of high-throughput image processing to capture rich SAM size variation within a diverse maize inbred panel. We demonstrate correlations between seedling SAM size and agronomically important adult traits such as flowering time, stem size and leaf node number. Combining SAM phenotypes with 1.2 million single nucleotide polymorphisms (SNPs) via genome-wide association study reveals unexpected SAM morphology candidate genes. Analyses of candidate genes implicated in hormone transport, cell division and cell size confirm correlations between SAM morphology and trait-associated SNP alleles. Our data illustrate that the microscopic seedling SAM is predictive of adult phenotypes and that SAM morphometric variation is associated with genes not previously predicted to regulate SAM size.

Original languageEnglish (US)
Article number8974
JournalNature communications
Volume6
DOIs
StatePublished - Nov 20 2015

Bibliographical note

Funding Information:
We thank K. Niklas (Cornell) for strategic advice in modelling SAMs as paraboloids. We thank C.T. (Eddy) Yeh (ISU) for assistance in SNP calling; and L. Coffey (ISU) for identifying the correlation between SAM size and flowering time. This work was funded by the National Science Foundation (NSF) grant IOS-1238142.

Publisher Copyright:
© 2015 Macmillan Publishers Limited. All rights reserved.

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