TY - JOUR
T1 - Genetic characterization of influenza A virus subtype H7N1 isolated from quail, Thailand
AU - Wongphatcharachai, Manoosak
AU - Wisedchanwet, Trong
AU - Nonthabenjawan, Nutthawan
AU - Jairak, Waleemas
AU - Chaiyawong, Supassama
AU - Bunpapong, Napawan
AU - Amonsin, Alongkorn
N1 - Publisher Copyright:
© 2014, Springer Science+Business Media New York.
PY - 2014/11/15
Y1 - 2014/11/15
N2 - In Thailand, surveillance for the highly pathogenic avian influenza virus H5N1 (HPAI-H5N1) has revealed high prevalence of the virus in quail in live-bird markets. This study monitored avian influenza viruses (AIVs) in quail farms in an area at high risk for HPAI-H5N1 over a 12-month period from 2009 to 2010. One-step real-time RT-PCR (rRT-PCR) results showed that 1.18 % of swab samples (24/2,040) were AIV positive. Among the rRT-PCR positive samples, three samples were identified as subtype H7N1. One Thai H7N1 virus designated “A/quail/Thailand/CU-J2882/2009 (H7N1)” was subjected to whole genome sequencing and genetic characterization. Phylogenetic analysis showed that the HA gene of the Thai H7N1 virus groups with those of the H7 Eurasian viruses. Interestingly, the NA gene of the virus was found to be closely related to those of the HPAI-H5N1 viruses from Vietnam and Thailand. This study constitutes the first report on AIV H7N1 in Thailand. Our results suggest the possibility of genetic reassortment between AIV-H7NX and HPAI-H5N1 in quail. The HA cleavage site of the Thai H7N1 virus contains no multiple amino acid insertions, suggesting low pathogenic characteristics for this virus.
AB - In Thailand, surveillance for the highly pathogenic avian influenza virus H5N1 (HPAI-H5N1) has revealed high prevalence of the virus in quail in live-bird markets. This study monitored avian influenza viruses (AIVs) in quail farms in an area at high risk for HPAI-H5N1 over a 12-month period from 2009 to 2010. One-step real-time RT-PCR (rRT-PCR) results showed that 1.18 % of swab samples (24/2,040) were AIV positive. Among the rRT-PCR positive samples, three samples were identified as subtype H7N1. One Thai H7N1 virus designated “A/quail/Thailand/CU-J2882/2009 (H7N1)” was subjected to whole genome sequencing and genetic characterization. Phylogenetic analysis showed that the HA gene of the Thai H7N1 virus groups with those of the H7 Eurasian viruses. Interestingly, the NA gene of the virus was found to be closely related to those of the HPAI-H5N1 viruses from Vietnam and Thailand. This study constitutes the first report on AIV H7N1 in Thailand. Our results suggest the possibility of genetic reassortment between AIV-H7NX and HPAI-H5N1 in quail. The HA cleavage site of the Thai H7N1 virus contains no multiple amino acid insertions, suggesting low pathogenic characteristics for this virus.
KW - Avian influenza virus
KW - H7N1
KW - Quail
KW - Thailand
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U2 - 10.1007/s11262-014-1120-6
DO - 10.1007/s11262-014-1120-6
M3 - Article
C2 - 25262289
AN - SCOPUS:84911454128
SN - 0920-8569
VL - 49
SP - 428
EP - 437
JO - Virus Genes
JF - Virus Genes
IS - 3
ER -