Genetic approaches in the study of heparan sulfate functions in drosophila

Masahiko Takemura, Hiroshi Nakato

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila has been a powerful system to explore the functions of HSPGs in vivo. In this chapter, we will introduce two genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting) and MARCM (mosaic analysis with a repressible cell marker).

Original languageEnglish (US)
Pages (from-to)497-505
Number of pages9
JournalMethods in Molecular Biology
Volume1229
DOIs
StatePublished - Jan 1 2015

Keywords

  • Drosophila melanogaster
  • GAL4/UAS
  • HSPG
  • MARCM
  • TARGET

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