Genetic and physiological characterization of oxytetracycline-resistant bacteria from giant prawn farms

Pimpak Heepngoen, Kannika Sajjaphan, John A. Ferguson, Michael J. Sadowsky

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Four hundred and thirteen oxytetracycline-resistant bacteria were recovered from six freshwater giant prawn farms with a history of oxytetracycline use. Most oxytetracycline-resistant isolates were Gram-negative bacteria. Six groups of oxytetracycline-resistant bacteria were classified using cluster analysis based on a comparison of levels of oxytetracycline resistance. Complex fingerprint patterns were obtained for 71 isolates studied. In general, the band patterns of isolates from different ponds were very similar, and the data indicated that the isolates were closely related. The exploration for cross-resistance found that most of the 71 oxytetracycline-resistant isolates were also resistant to tetracycline and chlortetracycline, but had a relatively low resistance to doxycycline. Many isolates showed higher chlortetracycline resistance than oxytetracycline resistance. Additionally, the oxytetracycline-resistant isolates were examined for the presence of tetracycline resistance (tet) genes. Fifty percent of the isolates carried one of the 14 known tet genes examined. The most common determinants were TetA and TetD. However, TetB, TetC, TetE, TetK, TetL, and TetM were also found with various frequencies.

Original languageEnglish (US)
Pages (from-to)199-206
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume18
Issue number2
StatePublished - Feb 28 2008

Keywords

  • Antibiotics
  • Freshwater giant prawn
  • Oxytetracycline-resistance bacteria

Fingerprint Dive into the research topics of 'Genetic and physiological characterization of oxytetracycline-resistant bacteria from giant prawn farms'. Together they form a unique fingerprint.

Cite this