Genetic and Phenotypic Characterization of Xanthomonas Species Pathogenic in Wheat in Uruguay

Felipe Clavijo, Rebecca D. Curland, Valentina Croce, Marıa I. Lapaz, Ruth Dill-Macky, Silvia Pereyra, Marıa I. Siri

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol with species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing was applied to examine the genetic diversity of X. translucens strains. Strains were assigned to four different sequence types, three of which were previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to X. prunicola and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota. In planta pathogenicity assays carried out on a BLS-susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, whereas non-translucens Xanthomonas sp. strains elicited an atypical symptom of dry necrosis. These findings suggest that local wheat fields are affected by X. translucens pv. undulosa and by a new wheat pathogen within the Xanthomonas genus.

Original languageEnglish (US)
Pages (from-to)511-520
Number of pages10
JournalPhytopathology
Volume112
Issue number3
DOIs
StatePublished - Mar 2022

Bibliographical note

Funding Information:
Funding: This study was financially supported by Agencia Nacional de Investigación e Innovación (research grant FCE_1_2017_1_135561) and Comisión Sectorial de Investigación Científica (research grant CSIC Grupos I+D_2000).

Publisher Copyright:
© 2022 The American Phytopathological Society

Keywords

  • Uruguay
  • Xanthomonas
  • bacterial leaf streak
  • bacterial pathogens
  • multilocus sequence analysis and typing
  • population biology
  • wheat

PubMed: MeSH publication types

  • Journal Article

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