TY - JOUR
T1 - Genetic and phenotypic characterization of a low-pathogenicity avian influenza H11N9 virus
AU - Li, Jinling
AU - Cardona, Carol J.
AU - Xing, Zheng
AU - Woolcock, Peter R.
PY - 2008/10/1
Y1 - 2008/10/1
N2 - An H11N9 low-pathogenicity avian influenza virus, A/duck/WA/663/97, was isolated from a sick Mandarin duck kept in an outdoor bird exhibit. Genetic and phenotypic characterization of the virus suggested that it originated from free-flying birds, a concept supported by genetic similarity with waterfowl isolates from the same geographic area and time period. This duck-origin virus had genetic features typical of H11 and N9 viruses, including no neuraminidase stalk deletion, no differences in putative glycosylation sites in either surface protein, and no addition of basic amino acid residues at the hemagglutinin cleavage site compared to published sequences. It replicated in both avian and mammalian cells in vitro, and experimentally challenged chickens developed mild acute upper respiratory lesions but no clinical signs of disease. It elicited immune responses in chickens, resulting in seroconversion in all infected birds, although antibody titers remained low over the experimental period.
AB - An H11N9 low-pathogenicity avian influenza virus, A/duck/WA/663/97, was isolated from a sick Mandarin duck kept in an outdoor bird exhibit. Genetic and phenotypic characterization of the virus suggested that it originated from free-flying birds, a concept supported by genetic similarity with waterfowl isolates from the same geographic area and time period. This duck-origin virus had genetic features typical of H11 and N9 viruses, including no neuraminidase stalk deletion, no differences in putative glycosylation sites in either surface protein, and no addition of basic amino acid residues at the hemagglutinin cleavage site compared to published sequences. It replicated in both avian and mammalian cells in vitro, and experimentally challenged chickens developed mild acute upper respiratory lesions but no clinical signs of disease. It elicited immune responses in chickens, resulting in seroconversion in all infected birds, although antibody titers remained low over the experimental period.
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U2 - 10.1007/s00705-008-0217-4
DO - 10.1007/s00705-008-0217-4
M3 - Article
C2 - 18825481
AN - SCOPUS:53849144615
VL - 153
SP - 1899
EP - 1908
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 10
ER -