Abstract
Experiments were conducted to determine the chromosomal location of the gene conditioning overproduction of a methionine-rich, 10-K zein in maize kernels of line BSSS53. In addition, the chromosomal location of the structural gene encoding the overproduced protein was determined. Whereas the structural gene, designated Zps10/(22), was found to be located on the long arm of chromosome 9 near the centromere, the locus regulating overproduction of the zein protein was mapped to the short arm of chromosome 4. This regulatory gene has been designated Zpr10/(22). Regulation of 10-K zein production by Zpr10/(22) is, therefore, via a trans-acting mechanism.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 761-767 |
| Number of pages | 7 |
| Journal | Theoretical and Applied Genetics |
| Volume | 78 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1 1989 |
Keywords
- Protein isoelectric focusing (IEF)
- Restriction fragment length polymorphism (RFLP) mapping
- Trans-acting factors
- Zea mays L.
- Zein