Genetic analysis of methionine-rich storage protein accumulation in maize

M. S. Benner, R. L. Phillips, J. A. Kirihara, J. W. Messing

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Experiments were conducted to determine the chromosomal location of the gene conditioning overproduction of a methionine-rich, 10-K zein in maize kernels of line BSSS53. In addition, the chromosomal location of the structural gene encoding the overproduced protein was determined. Whereas the structural gene, designated Zps10/(22), was found to be located on the long arm of chromosome 9 near the centromere, the locus regulating overproduction of the zein protein was mapped to the short arm of chromosome 4. This regulatory gene has been designated Zpr10/(22). Regulation of 10-K zein production by Zpr10/(22) is, therefore, via a trans-acting mechanism.

Original languageEnglish (US)
Pages (from-to)761-767
Number of pages7
JournalTheoretical and Applied Genetics
Volume78
Issue number6
DOIs
StatePublished - Dec 1 1989

Fingerprint

Zein
zein
storage proteins
Methionine
Zea mays
genetic techniques and protocols
methionine
structural genes
corn
Genes
chromosomes
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 4
Proteins
Centromere
centromeres
Regulator Genes
regulator genes
proteins
loci

Keywords

  • Protein isoelectric focusing (IEF)
  • Restriction fragment length polymorphism (RFLP) mapping
  • Trans-acting factors
  • Zea mays L.
  • Zein

Cite this

Genetic analysis of methionine-rich storage protein accumulation in maize. / Benner, M. S.; Phillips, R. L.; Kirihara, J. A.; Messing, J. W.

In: Theoretical and Applied Genetics, Vol. 78, No. 6, 01.12.1989, p. 761-767.

Research output: Contribution to journalArticle

Benner, M. S. ; Phillips, R. L. ; Kirihara, J. A. ; Messing, J. W. / Genetic analysis of methionine-rich storage protein accumulation in maize. In: Theoretical and Applied Genetics. 1989 ; Vol. 78, No. 6. pp. 761-767.
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