TY - JOUR
T1 - Generation of targeted retroviral vectors by using single-chain variable fragment
T2 - An approach to in vivo gene delivery
AU - Somia, Nikunj V.
AU - Zoppé, Monica
AU - Verma, Inder M.
PY - 1995/8/1
Y1 - 1995/8/1
N2 - We report the generation of a retroviral vector that infects human cells specifically through recognition of the low density lipoprotein receptor. The rationale for this targeted infection is to add onto the ecotropic envelope protein of Moloney murine leukemia virus, normally trophic for murine cells, a single-chain variable fragment derived from a monoclonal antibody recognizing the human low density lipoprotein receptor. This chimeric envelope protein was used to construct a packaging cell line producing a retroviral vector capable of high-efficiency transfer of the Escherichia coli β-galactosidase gene to human cells expressing low density lipoprotein receptor. This approach offers a generalized plan to generate cell and tissue-specific retroviral vectors, an essential step toward in vivo gene therapy strategies.
AB - We report the generation of a retroviral vector that infects human cells specifically through recognition of the low density lipoprotein receptor. The rationale for this targeted infection is to add onto the ecotropic envelope protein of Moloney murine leukemia virus, normally trophic for murine cells, a single-chain variable fragment derived from a monoclonal antibody recognizing the human low density lipoprotein receptor. This chimeric envelope protein was used to construct a packaging cell line producing a retroviral vector capable of high-efficiency transfer of the Escherichia coli β-galactosidase gene to human cells expressing low density lipoprotein receptor. This approach offers a generalized plan to generate cell and tissue-specific retroviral vectors, an essential step toward in vivo gene therapy strategies.
UR - http://www.scopus.com/inward/record.url?scp=0029145999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029145999&partnerID=8YFLogxK
U2 - 10.1073/pnas.92.16.7570
DO - 10.1073/pnas.92.16.7570
M3 - Article
C2 - 7638232
AN - SCOPUS:0029145999
SN - 0027-8424
VL - 92
SP - 7570
EP - 7574
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 16
ER -